Imaging-Based Resistance Assay Using Enhanced Luminescence-Tagged Reveals a Complex Epigenetic Network in Plant Defense Signaling Pathways.

High-throughput resistance assays in plants have a limited selection of suitable pathogens. In this study, we developed a strain chromosomally tagged with the Nanoluc luciferase (NL) from the deep-sea shrimp , a bioluminescent marker significantly brighter than the conventional firefly luciferase. Our reporter strain tagged with NL was more than 100 times brighter than tagged with the operon from , one of the existing luciferase-based strains.