In vitro efficacy of Metarhizium anisopliae sensu lato against unfed Amblyomma parvum (Acari: Ixodidae).

Amblyomma parvum Aragão (Acari: Ixodidae) is a tick species found with wide distribution in the Neotropical region. Even though it is a wildlife-related tick, it is also a frequent parasite of domestic animals, is aggressive to human beings and may harbor pathogenic microorganisms. Therefore, it is a target species for control on domestic animals, particularly those at the rural-wildlife interface.

Reaction of Bipolaris euphorbiae to environmental factors and its tolerance to solar and ultraviolet radiation.

The determination of the ideal cultivation conditions and the knowledge of solar radiation tolerance are important aspects that must be investigated for the use of Bipolaris euphorbiae Muchovej and Carvalho as a biocontrol agent. The present study was done to assess the growth rate, sporulation and viability of the fungus cultivated under different conditions of the initial pH value of the growth medium, temperature and photoperiod. The tolerance of this fungus' conidia to light from a source simulating solar and ultraviolet radiation was also assessed.

Effect of Metarhizium anisopliae fungus on off-host Rhipicephalus (Boophilus) microplus from tick-infested pasture under cattle grazing in Brazil.

This work aimed to assess the effect of the fungus Metarhizium anisopliae on off-host Rhipicephalus (Boophilus) microplus from tick-infested Brachiaria decumbens pasture undergoing cattle grazing. For this purpose a naturally tick-infested Brachiaria decumbens pasture of 60 m × 100 m with twelve grazing Holstein Friesian-Nelore (Bos indicus) cross breed bovines was sprayed 12 times, 21 days apart with an aqueous conidial suspension of the E9 isolate of M. anisopliae fungus.

Efficiency of entomopathogenic fungi in the control of eggs and larvae of the horn fly Haematobia irritans (Diptera: Muscidae).

The present study assessed the pathogenic effect of isolates E9, IBCB425 and IBCB159 of the Metarhizium anisopliae fungus, JAB06, JAB07 and AM09 of Beauveria bassiana, IBCB133 and CB75 of Isaria fumosorosea (=Paecilomyces fumosoroseus) and CG189 and CG195 of Isaria farinosa (=Paecilomyces farinosus) against eggs and larvae of the horn fly Haematobia irritans. Eggs were inoculated with suspensions containing 10(6), 10(7) and 10(8)conidiaml(-1) of the fungal isolates and observed after 48h to determine viability. In the larvae study, eggs were allowed to hatch into fresh bovine feces that had been treated with 10(8), 10(7) or 10(6)conidiamgfeces(-1).

Entomopathogenic fungal activity against pupae and adult Haematobia irritans (Diptera: Muscidae).

The horn fly Haematobia irritans is one of the most important ectoparasites associated with grazing bovines. This study investigated the pathogenic activity of Metarhizium anisopliae (E9, IBCB425 and IBCB159), Beauveria bassiana (JAB06, JAB07 and AM09), Isaria fumosorosea (=Paecilomyces fumosoroseus) (IBCB133 and CB75) and Isaria farinosa (=Paecilomyces farinosus) (CG189 and CG195) fungi isolates on pupae and adult H. irritans.

Susceptibility of adult and larval stages of the horn fly, Haematobia irritans, to the entomopathogenic fungus Metarhizium anisopliae under field conditions.

The efficacy of M. anisopliae strain E9 as a biological insecticide for the adult and larval stages of H. irritans was assessed under field conditions.