Identification and characterisation of antimicrobial compound produced by probiotic Alkalihalobacillus clausii 088AE.

In the present study, Alkalihalobacillus clausii 088AE was evaluated for the production of antimicrobial compounds, their characterisation, and identification. The results showed that, 48-h-old A. clausii supernatant is able to inhibit the growth of indicator bacteria Micrococcus luteus MTCC 106 (7.

Genetic and phenotypic assessments for the safety of probiotic 088AE.

In this study, we report on whole genome sequence analysis of clinically documented, commercial probiotic 088AE and genome features contributing to probiotic properties. The whole genome sequence of 088AE generated a single scaffold of 4,598,457 bp with 44.74 mol% G + C.

In Vitro Human Gastrointestinal Tract Simulation Systems: A Panoramic Review.

Simulated human gastrointestinal (GI) tract systems are important for their applications in the fields of probiotics, nutrition and health. To date, various in vitro gut systems have been available to study GI tract dynamics and its association with health. In contrast to in vivo investigations, which are constrained by ethical considerations, in vitro models have several benefits despite the challenges involved in mimicking the GI environment.

Impact of a Gastrointestinal Stable Probiotic Supplement LBSC on Human Gut Microbiome Modulation.

LBSC showed stability in acidic pH, bile and simulated human gastrointenstinal juices. Under static gut model, when passed through oral, gastric and intestinal phases, LBSC was found to be stable as free viable spores and also with various foods such as milk and baby foods, as well as American and European diets. In human studies, modulation of gut microbiota by LBSC was comprehended by whole genome metagenome analysis of fecal samples obtained from pre- and post-treatment of irritable bowel syndrome (IBS) patients.

Genome based safety assessment for Bacillus coagulans strain LBSC (DSM 17654) for probiotic application.

The present study on Bacillus coagulans strain LBSC (DSM 17654) describes the use of whole genome sequencing, in correlation with the phenotypic properties to assess the safety of the strain. Analysis of the 16S rRNA sequence of the B. coagulans strain LBSC (DSM 17654), showed 100% homology with 99% coverage with B.

A rapid, one step molecular identification of Trichoderma citrinoviride and Trichoderma reesei.

Trichoderma species are widely used as production hosts for industrial enzymes. Identification of Trichoderma species requires a complex molecular biology based identification involving amplification and sequencing of multiple genes. Industrial laboratories are required to run identification tests repeatedly in cell banking procedures and also to prove absence of production host in the product.