Structures, functions, and inhibitors of LUBAC and its related diseases.

Ubiquitination is a reversible posttranslational modification in which ubiquitin is covalently attached to substrates at catalysis by E1, E2, and E3 enzymes. As the only E3 ligase for assembling linear ubiquitin chains in animals, the LUBAC complex exerts an essential role in the wide variety of cellular activities. Recent advances in the LUBAC complex, including structure, physiology, and correlation with malignant diseases, have enabled the discovery of potent inhibitors to treat immune-related diseases and cancer brought by LUBAC complex dysfunction.

Comprehensive assessment of three typical antibiotics on cyanobacteria (Microcystis aeruginosa): The impact and recovery capability.

This innovative study provided a comprehensive evaluation of the effects of three typical antibiotics exposures (cefradine, norfloxacin and amoxicillin) on Microcystis aeruginosa in two periods (exposure and post-exposure) at a new perspective. The results indicated that the irreversible growth inhibition of M. aeruginosa attributed to the norfloxacin in the exposure and the re-exposure stages.

Occurrence, distribution and sources of bisphenol analogues in a shallow Chinese freshwater lake (Taihu Lake): Implications for ecological and human health risk.

Since the production and use of BPA were regulated in China around 2008, several bisphenol analogues were widely used to substitute BPA in the manufacture of polycarbonates, epoxy resins, and plastics. However, there is limited understanding of the spatial distribution, potential sources and risk assessment for those bisphenol analogues. In this study, seven bisphenol analogues were investigated in water and sediment samples from Taihu Lake, China.

[Establishment and application of co-transfection screening method for phytoestrogen active constituents].

Objective: To establish a highly sensitive screening method for phytoestrogen active constituents and to primarily screen the phytoestrogenic active constituents from the chickpea extractions by the method.

Method: Human ERalpha cDNA was cloned using MCF-7 total RNA as the template by RT-PCR and then was constructed into a pcDNA3 and named as pERalpha. The cell line MCF-7 was co-transfected with pERalpha and the reporter plasmid pERE-Luc which carrying the estrogen response element (ERE) plus the luciferase reporter gene.