Background: The etiology of early pregnancy loss is multifactorial and may include viral pathogens. The aim of this study was to investigate the presence of herpes simplex virus (HSV) in gestational tissues of first trimester pregnancy loss using molecular techniques.
Patients And Methods: Gestational tissue from 95 women with first trimester pregnancy loss and 36 women with elective termination of pregnancy was investigated by nested polymerase chain reaction (PCR) and in situ hybridization techniques.
Background: Viruses have been detected in atherosclerotic and non-atherosclerotic vascular tissues and may be involved in the mechanisms of atherogenesis. In the present study, we investigated the role of herpes simplex virus (HSV) in the early and late stages of coronary artery atherosclerosis.
Methods And Results: HSV prevalence was investigated in coronary artery samples from 42 autopsy cases, in which death was related to myocardial infarction (MI), and 28 young age autopsy cases without heart disease, who had died from fatal injuries (young victim group), using nested polymerase chain reaction (nPCR) and the highly sensitive in situ hybridization with tyramide signal amplification (ISH-TSA).
Objective: To investigate the prevalence of herpes simplex virus (HSV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in the semen of men with fertility problems.
Design: A descriptive clinical study.
Settings: Outpatient infertility clinic of a private hospital.