miR-21-3p and miR-192-5p in patients with type 2 diabetic nephropathy.

Objectives: Microribonucleic acids (microRNA/miRNA/miR-) are predicted to be useful in the early diagnosis, monitoring, and treatment of diabetic nephropathy (DN). We aimed to investigate the relationship of DN to miR-21-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-126-3p, miR-129-1-3p, miR-137, miR-192-5p, miR-212-3p, and miR-320c.

Methods: There were 50 healthy controls and 100 patients with type 2 diabetes mellitus (T2DM).

Assessment of estimated glomerular filtration rate based on cystatin C in diabetic nephropathy.

Introduction: GFR is estimated by using creatinine and cystatin C to determine renal dysfunction. Our aim was to evaluate estimated GFR (eGFR) based on cystatin C in type 2 diabetic patients with diabetic nephropathy (DN).

Methods: Study group included 52 controls (46% male, age: 54.

Assessment of Abbott Architect 25-OH vitamin D assay in different levels of vitamin D.

Background: There is increasing requests of Vitamin D test in many clinical settings in recent years. However, immunoassay performance is still a controversial topic. Several diagnostic manufacturers have launched automated 25-hydroxyvitamin D (25-OH D) immunoassays in the past decade.

Surrogate Biomarkers for Monitoring Healthcare Quality for Chronic Diseases Such as Diabetes Care.

Some laboratory tests or biomarkers are used as surrogate outcomes for health care effectiveness. HbA1c is defined as a surrogate biomarker since HbA1c values have been approved to be used in predictions of clinically important complications of diabetes mellitus. With the advance of information technology (IT) the real life data are aggregating as electronic health records (EHRs).

Which Skills are Needed and How They Should be Gained by Laboratory Medicine Professionals for Successful ISO 15189 Accreditation.

Clinical laboratories worldwide are accredited according to the "ISO standard, 15189:2012: Medical Laboratories-Requirements for Quality and Competence." Seeking accreditation has many challenges. Success requires the right competencies and knowledge and the right technical expert and trainer to lead the laboratory through the process.

A multicenter nationwide reference intervals study for common biochemical analytes in Turkey using Abbott analyzers.

Background: A nationwide multicenter study was organized to establish reference intervals (RIs) in the Turkish population for 25 commonly tested biochemical analytes and to explore sources of variation in reference values, including regionality.

Methods: Blood samples were collected nationwide in 28 laboratories from the seven regions (≥400 samples/region, 3066 in all). The sera were collectively analyzed in Uludag University in Bursa using Abbott reagents and analyzer.

Comparison of cetylpyridinium chloride and cetyltrimethylammonium bromide extractive procedures for quantification and characterization of human urinary glycosaminoglycans.

Background: Glycosaminoglycans (GAGs) are natural complex polysaccharides that are important in several pathological processes. Urinary GAGs have long been investigated for their possible modifications in many pathological conditions. In some cases, they have been found to have diagnostic utility.

Rate of T alleles and TT genotype at MTHFR 677C->T locus or C alleles and CC genotype at MTHFR 1298A->C locus among healthy subjects in Turkey: impact on homocysteine and folic acid status and reference intervals.

Methylenetetrahydrofolate reductase (MTHFR) is important for folate and homocysteine (Hcy) metabolism. MTHFR 677C->T and 1298A->C MTHFR are two most common mutations which can affect folate and total homocysteine (tHcy) status. This study was designed to determine the rate of MTHFR 677C->T and 1298A->C mutations, and their influence on serum folate, Hcy and vitamin B12 status and the reference intervals in 402 healthy Turkish adults.

Technical and clinical evaluation of a glucose meter employing amperometric biosensor technology.

The objective of this study was to assess accuracy and precision of the Optimum H System for measuring glucose in fresh venous whole blood samples. Ninety-one whole blood specimens were analyzed duplicate on two optimum blood glucose meter and compared to YSI reference analyser and Beckman LX20 laboratory analyser. The study demonstrated that the Optimum H System gives clinically accurate, plasma equivalent glucose results for venous samples, compared to the YSI*1.

Use of total patient data for indirect estimation of reference intervals for 40 clinical chemical analytes in Turkey.

In the present study we used patient data to calculate laboratory-specific indirect reference intervals. These values were compared with reference intervals obtained for a healthy group according to recommendations of the International Federation of Clinical Chemistry and Laboratory Medicine and manufacturer suggestions. Laboratory results (422,919 records) from all subjects of 18-45 years of age over a 1-year period were retrieved from our laboratory information system and indirect reference intervals for 40 common analytes were estimated using a modified Bhattacharya procedure.

Role of free radicals in peptic ulcer and gastritis.

Background/aims: It has been suggested that the free radicals are closely related with peptic ulcer disease and gastritis. Although many studies have been undertaken to clarify the role of oxygen-derived free radicals, most of them were carried out in animal models. The aim of this study was to assess the reactive oxygen species activity and the damage in Helicobacter pylori-infected gastric mucosa in humans.

Effect of α-Tocopherol on Lipid Peroxidation Caused by Cisplatin in Rat Kidney.

Cisplatin (CDDP) is one of the most commonly used antineoplastic agents in current clinical practice. The major toxicities of CDDP are nonhaematological as nephrotoxicity and ototoxicity. Free oxygen radicals are known to play major role in CDDP-induced acute renal failure in rats.