Probiotic supplement for the treatment of polycystic ovarian syndrome.

Polycystic Ovarian Syndrome is one of the major prevalent causes of infertility reported worldwide nearly 6-26 %, especially in girls hitting puberty and women at their childbearing age. The main clinical manifestations include irregular menstrual cycle, small cysts on one or both ovaries, chronic oligo-anovulation, and hirsutism. The etiological criteria are very complex and related to many factors like obesity, insulin sensitivity, inflammation, hyperandrogenism, diabetes mellitus type II, cardiovascular diseases, and dysbiosis of gut microbiota.

Isolation, characterization, and cloning of thermostable pullulanase from ADM-11.

This study aimed to identify thermo-stable pullulanase-producing bacteria in soil samples of potato fields and food-producing companies. Pullulan agar medium was used to screen 17 bacterial strains, which were incubated at 65 °C. The isolate with the maximum activity (375U/ml) was selected and recognized as ADM-11 by morphological, biochemical characterization, and 16S rRNA gene sequencing.

Identification of arsenic oxidizing genes fragment in sp. strain 1S1 and its cloning in (DH5).

sp. strain 1S1, an arsenic-resistant bacterial strain, was isolated with 75 mM MIC against arsenite. Brownish precipitation with silver nitrate appeared, which confirmed its oxidizing ability against arsenite.

Effect of probiotics on hematological parameters of male and female Wistar rats.

In the present study, the effect of probiotics on the hematology of Wistar rats was examined. Locally isolated MZ707748 (Pro 1) MZ710117 (Pro 2) MZ727611 (Pro 3), and MZ735961 (Pro 4) were used One strain of probiotic, 14 (Pro 5), was purchased commercially. Different groups were designed as G1, G2, G3, G4, and 5, G5/PC consisting only pro 5 and NC & 0 day were untreated.

sp. strain CS2: A potential candidate for arsenic bioremediation from industrial wastewater.

A multiple metal-resistant sp. strain CS2, isolated from an industrial wastewater, resisted arsenate and arsenate upto 280 and 40 mM. The order of resistance against multiple metals was Arsenate > Arsenite > Selenium = Cobalt > Lead = Nickel > Cadmium = Chromium = Mercury.

Uptake of lead by bacteria isolated from industrial effluents and their potential use in bioremediation of wastewater.

Due to rising populations and human activities, heavy metals (HM) toxicity has become a serious problem for all life forms. The present study deals with isolating and identifying lead-resistant bacteria from contaminated wastewater of tanneries effluents. Two isolated strains were identified as (ID1), and sp (ID3), and both strains resisted a 25 mM concentration of Lead nitrate (Pb (NO)).

Evaluation of isolated probiotics on the efficacy of immune system in male and female Wistar rats.

Probiotics were isolated from fruits and vegetables. Microscopic, biochemical, and molecular tests were carried out for the characterization of strains of probiotics. To assess the effects of isolated probiotics on immunity, male and female (15 + 15) Wistar rats were randomly distributed into 5 groups: 0-day, negative control, positive control (commercially available Lactobacillus acidophilus-14), laboratory isolated probiotics with accession numbers; Lactobacillus plantarum (MZ707748) and Lactobacillus plantarum (MZ729681), respectively.

Characterization of plastic degrading bacteria isolated from sewage wastewater.

Plastic is a fundamental polymer used in routine life and disposed of in sewage. It leads to microplastic pollution in aquatic organisms, introducing it into the food chain and affecting human health. In the present study, samples were collected from sewage wastewater to isolate the bacteria that could potentially reduce plastic.

Biotoxicity comparison of to control vector borne diseases against mosquito fauna.

The current study was designed to evaluate the biotoxicity of screened echo-friendly strains from different areas of Pakistan. Out of 50 samples, 36% isolates were quarantined from soil containing cattle waste after morphological, biochemical, and molecular characterization. The toxicity bioassays with spores and protein diet proved that 11 isolates were utmost noxious to 3rd instar larvae of mosquitoes and .

Biotoxicity assessment of cloned cry 11 protein gene from 9NF.

The current investigation describes the isolation and characterization of toxic . local isolates harboring 99% homology with prototoxin (AXJ97553.1 and novel OUB27301.