Publications by authors named "Dilana Elisabeth Staudt"
Aim: To evaluate the impact of CYP3A4*22 in the formation of endoxifen (EDF) and hydroxytamoxifen (HTF), under different CYP2D6 genotypic backgrounds.
Materials & Methods: 178 patients were enrolled in the study. CYP2D6 and CYP3A4 genotyping and tamoxifen (TAM) and metabolites quantification were performed.
Article Synopsis
- * The method takes only 8 minutes for analysis, with high precision (CV % < 10.75%) and accuracy (94.5% to 110.3%), achieving recoveries between 40% to 92% from blood spots.
- * It demonstrated strong correlation with plasma concentrations, effectively identifying patients with low drug levels that could indicate a need for treatment adjustment, especially relevant for optimizing breast cancer therapy in low-resource settings.
Objective: To develop and validate a method for determination of dextromethorphan (DMT) and dextrorphan (DTP) in plasma samples using HPLC-FL and to apply it to CYP2D6 phenotyping of a population from the South of Brazil.
Methods: Samples were prepared by hydrolysis and liquid-liquid extraction. Analysis was conducted in a reversed phase column, with isocratic elution and fluorescence detection.