Transgenerational disruption of functional 5-HTR-induced connectivity in the adult mouse brain by traumatic stress in early life.

Traumatic stress in early life is a strong risk factor for psychiatric disorders that can affect individuals across several generations. Although the underlying mechanisms have been proposed to implicate serotonergic transmission in the brain, the neural circuits involved remain poorly delineated. Using pharmacological functional magnetic resonance imaging in mice, we demonstrate that traumatic stress in postnatal life alters 5-HT receptor-evoked local and global functions in both, the exposed animals and their progeny when adult.

Multimodal imaging of pancreatic beta cells in vivo by targeting transmembrane protein 27 (TMEM27).

Aims/hypothesis: Non-invasive diagnostic tools specific for pancreatic beta cells will have a profound impact on our understanding of the pathophysiology of metabolic diseases such as diabetes. The objective of this study was to use molecular imaging probes specifically targeting beta cells on human samples and animal models using state-of-the-art imaging modalities (fluorescence and PET) with preclinical and clinical perspective.

Methods: We generated a monoclonal antibody, 8/9-mAb, targeting transmembrane protein 27 (TMEM27; a surface N-glycoprotein that is highly expressed on beta cells), compared its expression in human and mouse pancreas, and demonstrated beta cell-specific binding in both.

In vivo precision of bootstrap algorithms applied to diffusion tensor imaging data.

Purpose: To determine the precision for in vivo applications of model and non-model-based bootstrap algorithms for estimating the measurement uncertainty of diffusion parameters derived from diffusion tensor imaging data.

Materials And Methods: Four different bootstrap methods were applied to diffusion datasets acquired during 10 repeated imaging sessions. Measurement uncertainty was derived in eight manually selected regions of interest and in the entire brain white matter and gray matter.

Hybrid small animal imaging system combining magnetic resonance imaging with fluorescence tomography using single photon avalanche diode detectors.

The high sensitivity of fluorescence imaging enables the detection of molecular processes in living organisms. However, diffuse light propagation in tissue prevents accurate recovery of tomographic information on fluorophore distribution for structures embedded deeper than 0.5 mm.