Synchronization of spontaneous otoacoustic emissions to a 2f1-f2 distortion product.

Synchronization of spontaneous otoacoustic emissions to a cubic distortion frequency fs = 2f1-f2 has been studied. Stimulus, consisting of two primary tones at frequency f1 and f2, could easily be filtered out of the microphone signal. This enabled us to monitor emission phase with respect to synchronization frequency fs, by recording zero-crossing moments of the microphone signal.

Temperature effects on auditory nerve fiber response in the American bullfrog.

Single fiber recordings were made from auditory nerve fibers of the American bullfrog (Rana catesbeiana). As temperature was raised: (1) Best frequencies of fibers from the amphibian papilla (N = 15) increased. Below 600 Hz best frequency changes up to 0.

A simple method to change geometrical proportions of muscles by means of a tibial lengthening osteotomy in the rat.

A method is designed to change geometrical proportions of muscles in the rat. After a tibial osteotomy, the application of a stainless steel prosthesis resulted in an elongation of about 8% of the tibia. In 23 out of 28 operated rats a correct application of the prosthesis was maintained till the rats were sacrificed.

Blocking by anti-idiotypic antibodies of monoclonal antibody mediated protection in mice against encephalomyocarditis virus induced diabetes and lethal disease.

Monoclonal antibodies (MA) UM 21.1 and UM 21.2 protect mice against encephalomyocarditis virus (D-variant) induced diabetes and lethal disease.

Assignment of seven genes to distinct intervals on the midportion of human chromosome 19q surrounding the myotonic dystrophy gene region.

Hybridization studies using a panel of somatic cell hybrids with subchromosomal segments of 19q have localized the genes encoding hormone-sensitive lipase (LIPE), carcinoembryonic antigen (CEA), and small nuclear ribonucleoprotein polypeptide A (SNRPA) to various regions of 19q13.1; the cellular receptor for poliovirus sensitivity (PVS) to 19q13.2; and the genes coding for prostate-specific antigen (APS), human pancreatic kallikrein (KLK1), and small nuclear ribonucleoprotein 70-kD polypeptide (SNRP70) to 19q13.

Magnetic resonance imaging of the cerebral aqueduct. Signal intensity time curves demonstrated by fast acquisition with multiple excitation (FAME).

Using cardiac-gated fast acquisition with multiple excitation (FAME), time curves of the cerebral aqueduct signals were derived in 19 healthy volunteers and 14 patients. A mean curve of the normal subjects was determined during systole. A relatively stable point of time was found at 270 msec after the R-wave supposed to be the reversal of the flow of cerebral-spinal fluid in the aqueduct.

Spontaneous otoacoustic emissions in the European edible frog (Rana esculenta): spectral details and temperature dependence.

Spontaneous otoacoustic emissions were recorded in 41 ears of 29 European edible frogs (Rana esculenta). Emission frequencies ranged from 450 to 1350 Hz. The distribution of frequencies shows two distinct populations: one above and one below 1 kHz.

Synthesis of heterocyclic N-acetoxyarylamines and their reactivity with DNA.

2-Acetoxyamino-5-phenylpyridine and 2-acetoxyamino-3-methyl-5-phenylpyridine, being proposed ultimate carcinogens of the heterocyclic aromatic amines 2-amino-5-phenylpyridine (APP) and 2-amino-3-methyl-5-phenylpyridine (AMPP), respectively, were synthesized, crystallized and characterized. Using the 32P-postlabelling technique, we show that the total amount of adducts found in DNA after reaction with these N-acetoxyarylamines is at least 30- and 450-fold higher than in DNA reacted with equimolar amounts of the proposed proximate carcinogens 2-hydroxyamino-5-phenylpyridine and 2-hydroxyamino-3-methyl-5-phenylpyridine, respectively. These results support a postulated activation mechanism, in which N-acetoxyarylamines are the ultimate reactive species responsible for DNA modification by carcinogenic aromatic amines in vivo.

DC injection alters spontaneous otoacoustic emission frequency in the frog.

A permanent wire electrode was implanted in the inner ear of the green frog (Rana esculenta). Through this electrode direct current was delivered to the inner ear fluid of the frog, during recording of a spontaneous otoacoustic emission (SOAE). Both SOAE frequency and amplitude turned out to depend on DC level and polarity.

Regional localization of the gene encoding pregnancy specific beta-1-glycoprotein 1 (PSBG1) to human chromosome 19q13.1.

Pregnancy specific beta-1-glycoprotein (PSBG), a major product of the human placenta, is encoded by multiple genes. Southern blot hybridization of human x rodent somatic cell hybrid DNAs with a cDNA specific for one member of the PSBG gene family allowed us to map this gene to human chromosome 19. Further analysis using hybrids with subchromosomal segments of 19q revealed that the gene maps to the distal segment of region 19q13.

Hypnoanalgesia with R 8110/fentanyl in the dog: pharmacodynamic and pharmacokinetic interactions.

The pharmacokinetics and clinical effects of the short-acting hypnotic R 8110 and of the narcotic analgesic fentanyl were studied in the dog. The effects of separate intravenous (i.v.

Temperature dependence of frog spontaneous otoacoustic emissions.

Spontaneous otoacoustic emissions are recorded from frog ears at various body temperatures. Frequency spectra and amplitude distributions of emissions are determined. Both power and frequency of emission signals show a strong temperature dependence.

Comparison of the effects of etomidate and its fluoro analogue, R 8110, on plasma cortisol, 11 beta-deoxycortisol, 17 alpha-hydroxyprogesterone and testosterone concentrations in dogs.

R 8110, an imidazole derivative, was shown to be clinically superior to etomidate for induction and maintenance of anaesthesia in dogs. The present study compared the effects of intravenous (i.v.

Clinical, cardiovascular and respiratory effects of R8110 in premedicated dogs.

The clinical, cardiovascular and respiratory effects after i.v. administration of R8110, a fluoro analogue of etomidate (Fig.

Quality improvement of patient care: some preliminary results of a hospital-wide quality-assurance project at the University Hospital of Maastricht.

Dutch hospitals have to design their quality-assessment programmes within the boundaries set by "indirect government legislation". At the University hospital of Maastricht a five-stage project was started in 1985 to construct a hospital-wide quality-assurance organisation that includes regular review of the quality of professional performance. This paper will describe intermediate results of the first stage and will discuss the results of different subsurveys undertaken to assess the current level of quality of care and several key-issues conditional to the implementation of such a hospital-wide structure in the existing organisation of a University hospital.

R 8110, a new short-acting hypnotic in dogs.

The clinical, respiratory and cardiovascular effects of intravenous injections of R 8110, a fluor analogue of etomidate, were studied in unpremedicated dogs. Clinical observations were carried out after intravenous injections of 3 and 4 mg kg-1 of R 8110. Cardiovascular studies were conducted after an intravenous injection of 3 mg kg-1.

Multi-exponential relaxation analysis with MR imaging and NMR spectroscopy using fat-water systems.

This study was undertaken to evaluate the feasibility of multiexponential relaxation data analysis to MR imaging techniques. The first part of this study contains accurate relaxation time measurements performed on a conventional spectrometer. In the second part, essentially the same measuring techniques were applied but now on standard whole body MR imaging equipment.

The occurrence of click-evoked oto-acoustic emissions ("Kemp echoes") in normal-hearing ears.

Altogether 210 normal-hearing ears were screened regarding the possibility of producing click-evoked oto-acoustic emissions ("Kemp echoes"). Only a limited number of these ears displayed an emission. This finding limits the useful application of this technique as a method to define cochlear functioning.

The use in practice of inactivated oil emulsion vaccine against infectious bursal disease in broiler breeders and its influence on the progeny: a comparative field trial.

Field trials were conducted to establish the effect of the use of an inactivated oil emulsion vaccine against Infectious Bursal Disease (IBD OEV) in broiler breeder hens, and its effects on their progeny. The performance of 18 broiler flocks, which were the progeny of the IBD OEV vaccinated breeder hens, but which were not vaccinated with a live vaccine against IBD, was equal to that of broiler flocks which were vaccinated with a live IBD vaccine and originated from parent stock that had been vaccinated only against IBD with a live vaccine. In none of the 18 flocks, progeny of IBD OEV vaccinated parents, was IBD diagnosed.

Derivation of quantitative information in NMR imaging: a phantom study.

The use of NMR imaging as a quantitative research tool requires insight into the relationship between various imaging techniques and their resultant images. Work was undertaken to elucidate this relationship by using the following procedure. First, a theoretical model of NMR imaging under various pulse sequences was elaborated.

Direct cardiac NMR imaging of heart wall and blood flow velocity.

Nuclear magnetic resonance (NMR) imaging is used to produce in the same scan both anatomical and functional information of the heart and great vessels. A method is described to generate velocity images by the use of phase shifts for moving spins induced by imaging gradients under electrocardiogram (ECG) synchronized imaging conditions. The influence of the different gradients is discussed together with methods to obtain velocity information for each gradient direction separately.

Restoration of signal polarity in a set of inversion recovery NMR images.

A method is described which enables unambiguous retrieval of sign information in a set of magnetic resonance magnitude images of the inversion recovery type. The proposed method starts from the observation that the inversion recovery curve S is a monotonically increasing function of the inversion time TI, and comes down to finding the zero-crossing time TI0 of this curve for each pixel within the image. Absolute Value(S)and S are then related by S(TI) = -Absolute Value(S(TI)) for TI =/< TI(0) and S(TI) = + Absolute Value(S(TI)) for TI =/> TI0.