Quantification of hydroxyl radicals (˙OH), one form of reactive oxygen species (ROS), plays critical roles in early diagnosis and treatment monitoring of various diseases. In this work, we report the development of a responsive nanoprobe for ratiometric fluorescence detection and imaging of ˙OH in macrophage polarization. The nanoprobe, BSA-CCA@LDH-SRB, was designed and prepared using coumarin 3-carboxylic acid (CCA) as the sensing unit for ˙OH, and sulforhodamine B (SRB) loaded on layered double hydroxide (LDH) served as the fluorescent reference component.
View Article and Find Full Text PDFThe anaerobic oxidation of methane (AOM) carried out by anaerobic methanotrophic archaea (ANME) plays an important role in mitigating methane emissions from aqueous environments and has applications in bioremediation and wastewater treatment. Previous studies showed that AOM could be coupled to chromate reduction. However, the specific responsible microorganisms and the biochemical mechanisms are unclear.
View Article and Find Full Text PDFThe development of reliable probe technology for the detection of bisulfite (HSO) in situ in food and biological samples is contributing significantly to food quality and safety assurance as well as community health. In this work, a responsive probe, EHDI, is developed for ratiometric fluorescence detection of HSO in aqueous solution, meat samples, and living cells. The probe is designed based on the HSO triggered 1,4-addition of electron deficit C = C bond of EHDI.
View Article and Find Full Text PDFHydroxyl radical (•OH), a highly reactive oxygen species (ROS), is assumed as one of the most aggressive free radicals. This radical has a detrimental impact on cells as it can react with different biological substrates leading to pathophysiological disorders, including inflammation, mitochondrion dysfunction, and cancer. Quantification of this free radical in-situ plays critical roles in early diagnosis and treatment monitoring of various disorders, like macrophage polarization and tumor cell development.
View Article and Find Full Text PDFThe development of highly active acid-base catalysts for transfer hydrogenations of biomass derived carbonyl compounds is a pressing challenge. Solid frustrated Lewis pairs (FLP) catalysis is possibly a solution, but the development of this concept is still at a very early stage. Herein, stable, phase-pure, crystalline hydrotalcite-like compounds were synthesized by incorporating cerium cations into layered double hydroxide (MgAlCe-LDH).
View Article and Find Full Text PDFWe report the development of a red-emitting fluorescence probe (XDS) for hydrogen sulfide (HS) detection in biosystems, real-world food samples, and application of this probe for monitoring of HS production during food spoilage. The XDS probe is developed by coupling of coumarin derivative to rhodanic-CN through a HS responsive CC bond. Remarkable fluorescence quenching of XDS is observed as a result of the response to HS.
View Article and Find Full Text PDFLuminescence chemosensors are one of the most useful tools for the determination and imaging of small biomolecules and ions in situ in real time. Based on the unique photo-physical/-chemical properties of ruthenium(II) (Ru(II)) complexes, the development of Ru(II) complex-based chemosensors has attracted increasing attention in recent years, and thus many Ru(II) complexes have been designed and synthesized for the detection of ions and small biomolecules in biological and environmental samples. In this work, we summarize the research advances in the development of Ru(II) complex-based chemosensors for the determination of ions and small biomolecules, including anions, metal ions, reactive biomolecules and amino acids, with a particular focus on binding/reaction-based chemosensors for the investigation of intracellular analytes' evolution through luminescence analysis and imaging.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
March 2021
Hydrogen peroxide (HO), depending on its levels, plays a crucial role in either modulating various biological processes as a signal molecule, or mediating oxidative damage as a toxin. Therefore, monitoring intracellular HO levels is pivotal for exploring its physiological and pathological roles. Using a modified 2-(2'-hydroxyphenyl) benzothiazole (HBT) as the fluorophore, and a pinacol phenylborate ester as the responsive group, herein we developed an excited-state intramolecular proton transfer (ESIPT)-based probe BTFMB.
View Article and Find Full Text PDFA rationally designed near-infrared two-photon fluorescent probe () for selectively detecting cysteine (Cys) has been developed based on a newly designed conjugation-enhanced 2-(2'-hydroxyphenyl)benzothiazole derivative as the fluorophore, an acrylate moiety as the Cys reaction site, and an -methylpyridinium scaffold as both the unit of organelle targeting and improving water solubility. The probe alone essentially emitted no fluorescence, whereas it achieved a superb near-infrared fluorescence emission (713 nm) enhancement within 15 min with a significant Stokes shift (302 nm) in the presence of Cys. The photoluminescence mechanism of the probe toward Cys was modulated by excited-state intramolecular proton transfer (ESIPT) and intramolecular charge transfer (ICT) processes.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
September 2020
Glutathione (GSH), an extremely important antioxidant, is a major participant in maintaining redox homeostasis and tightly associated with various clinical diseases. Thus, accurate and rapid detection of intracellular GSH is imperative to elucidate its role in physiological and pathological processes. Herein, by modifying 2-(2'-hydroxyphenyl) benzothiazole (HBT) scaffold, we developed an excited-state intramolecular proton transfer (ESIPT)-based fluorescent probe BTFMD for tracking GSH, which exhibited good selectivity, excellent water solubility, a large Stokes shift (181 nm) and fast response rate (within 10 min).
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