Publications by authors named "Digamber Singh Chahar"
Article Synopsis
- The Rapid Fluorescence Focus Inhibition Test (RFFIT) measures the potency of rabies monoclonal antibodies, but is time-consuming and requires specialized facilities and trained personnel.
- The World Health Organization has encouraged the development of alternative methods, leading to the creation of an In-vitro ELISA test that uses inactivated rabies vaccine to quantify antibody potency.
- This new ELISA test is validated for accuracy and sensitivity, easy to perform, cost-effective, and can be conducted without the need for specialized labs, providing results in just a few hours.
Current postexposure prophylaxis of rabies includes vaccines, human rabies immunoglobulin (RIG), equine RIG, and recombinant monoclonal antibodies (mAb). In the manufacturing of rabies recombinant mAb, charge variants are the most common source of heterogeneity. Charge variants of rabies mAb were isolated by salt gradient cation exchange chromatography (CEX) to separate acidic and basic and main charge variants.
View Article and Find Full Text PDFWith the advancements in upstream technologies, the capacity for monoclonal antibody (mAb) production has transformed from a few milligrams to grams per liter. These titers lead to enormous pressure on downstream processes (DSPs), which need to be reworked to achieve higher efficiency and better utilization of available resources. Various parameters, such as column sizing, aggregate removal, filtration and volume handling, must be considered while designing a facility for commercial scale.
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