Publications by authors named "Diego Vargas-Blanco"
Diverse intrinsic properties shape transcript stability and stabilization in .
NAR Genom Bioinform
September 2024
Article Synopsis
- Mycobacteria manage their mRNA stability to adapt to environmental stresses, but the specific mechanisms for this regulation aren't well understood.
- In a study, the researchers measured the half-lives of mRNA across different growth conditions and found that hypoxia led to increased global stabilization of transcripts, especially for essential genes.
- They also created machine learning models to analyze the impact of various transcript properties on stability, revealing that these properties differ based on growth conditions and whether the transcripts have leaders or not.
Inhibition of Bruton's tyrosine kinase (BTK) through covalent modifications of its active site (e.g., ibrutinib [IBT]) is a preferred treatment for multiple B cell malignancies.
View Article and Find Full Text PDFThe host type I interferon (IFN) pathway is a major signature of inflammation induced by the human fungal pathogen, Candida albicans. However, the molecular mechanism for activating this pathway in the host defence against C. albicans remains unknown.
View Article and Find Full Text PDFArticle Synopsis
- * RNase E affects the degradation rates of transcripts from around 89% of protein-coding genes in Mycolicibacterium smegmatis, with a more significant impact on leadered transcripts compared to leaderless ones.
- * The researchers found that RNase E has a preference for cleaving RNA in C-rich regions and identified precise cleavage sites in Mycobacterium tuberculosis, highlighting RNase E's significant influence on its transcriptome.
Aspergillus fumigatus is a ubiquitous environmental mold that causes significant mortality particularly among immunocompromised patients. The detection of the -derived carbohydrate galactomannan in patient serum and bronchoalveolar lavage fluid is the major biomarker used to detect A. fumigatus infection in clinical medicine.
View Article and Find Full Text PDFBacteria have a remarkable ability to sense environmental changes, swiftly regulating their transcriptional and posttranscriptional machinery as a response. Under conditions that cause growth to slow or stop, bacteria typically stabilize their transcriptomes in what has been shown to be a conserved stress response. In recent years, diverse studies have elucidated many of the mechanisms underlying mRNA degradation, yet an understanding of the regulation of mRNA degradation under stress conditions remains elusive.
View Article and Find Full Text PDFArticle Synopsis
- Regulation of gene expression is crucial for mycobacteria to manage stress during infections and environmental challenges.
- Mycobacteria primarily use leaderless transcripts, which have been less studied compared to leadered transcripts, and their effects on mRNA stability and translation efficiency remain unclear.
- Experiments revealed that the 5' UTR of a specific transcript impacts its mRNA stability and translation rates differently than synthetic counterparts, while leaderless transcripts showed comparable translation efficiency but lower production rates.
The success of as a human pathogen is due in part to its ability to survive stress conditions, such as hypoxia or nutrient deprivation, by entering nongrowing states. In these low-metabolism states, can tolerate antibiotics and develop genetically encoded antibiotic resistance, making its metabolic adaptation to stress crucial for survival. Numerous bacteria, including , have been shown to reduce their rates of mRNA degradation under growth limitation and stress.
View Article and Find Full Text PDFA pre-therapeutic coating for medical devices that prevents the attachment of Candida albicans.
Ann Clin Microbiol Antimicrob
May 2017
Background: Hospital acquired fungal infections are defined as "never events"-medical errors that should never have happened. Systemic Candida albicans infections results in 30-50% mortality rates. Typically, adhesion to abiotic medical devices and implants initiates such infections.
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