BIFROST: A method for registering diverse imaging datasets of the brain.

Imaging methods that span both functional measures in living tissue and anatomical measures in fixed tissue have played critical roles in advancing our understanding of the brain. However, making direct comparisons between different imaging modalities, particularly spanning living and fixed tissue, has remained challenging. For example, comparing brain-wide neural dynamics across experiments and aligning such data to anatomical resources, such as gene expression patterns or connectomes, requires precise alignment to a common set of anatomical coordinates.

Flexible circuit mechanisms for context-dependent song sequencing.

Sequenced behaviours, including locomotion, reaching and vocalization, are patterned differently in different contexts, enabling animals to adjust to their environments. How contextual information shapes neural activity to flexibly alter the patterning of actions is not fully understood. Previous work has indicated that this could be achieved via parallel motor circuits, with differing sensitivities to context.

BIFROST: a method for registering diverse imaging datasets of the brain.

Unlabelled: The heterogeneity of brain imaging methods in neuroscience provides rich data that cannot be captured by a single technique, and our interpretations benefit from approaches that enable easy comparison both within and across different data types. For example, comparing brain-wide neural dynamics across experiments and aligning such data to anatomical resources, such as gene expression patterns or connectomes, requires precise alignment to a common set of anatomical coordinates. However, this is challenging because registering functional imaging data to reference atlases requires accommodating differences in imaging modality, microscope specification, and sample preparation.

Neural network organization for courtship-song feature detection in Drosophila.

Animals communicate using sounds in a wide range of contexts, and auditory systems must encode behaviorally relevant acoustic features to drive appropriate reactions. How feature detection emerges along auditory pathways has been difficult to solve due to challenges in mapping the underlying circuits and characterizing responses to behaviorally relevant features. Here, we study auditory activity in the Drosophila melanogaster brain and investigate feature selectivity for the two main modes of fly courtship song, sinusoids and pulse trains.

Thermoregulatory, behavioral and productive responses of laying hens supplemented with different types and dosages of phytases raised in a hot environment: An integrative approach.

This study had the following objectives: (i) to evaluate the thermoregulatory and behavioral responses of light laying hens supplemented with different types and dosages of phytases in the two day shifts; and (ii) to integrate the thermoregulatory and behavioral responses with performance of these birds raised in a hot environment. 270 light laying hens of the Hy-Line White lineage, with a body weight of 1.60 ± 0.

Auditory activity is diverse and widespread throughout the central brain of Drosophila.

Sensory pathways are typically studied by starting at receptor neurons and following postsynaptic neurons into the brain. However, this leads to a bias in analyses of activity toward the earliest layers of processing. Here, we present new methods for volumetric neural imaging with precise across-brain registration to characterize auditory activity throughout the entire central brain of Drosophila and make comparisons across trials, individuals and sexes.

The neural basis for a persistent internal state in females.

Sustained changes in mood or action require persistent changes in neural activity, but it has been difficult to identify the neural circuit mechanisms that underlie persistent activity and contribute to long-lasting changes in behavior. Here, we show that a subset of Doublesex+ pC1 neurons in the female brain, called pC1d/e, can drive minutes-long changes in female behavior in the presence of males. Using automated reconstruction of a volume electron microscopic (EM) image of the female brain, we map all inputs and outputs to both pC1d and pC1e.

Discovery of a New Song Mode in Drosophila Reveals Hidden Structure in the Sensory and Neural Drivers of Behavior.

Deciphering how brains generate behavior depends critically on an accurate description of behavior. If distinct behaviors are lumped together, separate modes of brain activity can be wrongly attributed to the same behavior. Alternatively, if a single behavior is split into two, the same neural activity can appear to produce different behaviors.

Functional role of long-lived flowers in preventing pollen limitation in a high elevation outcrossing species.

Low pollinator visitation in harsh environments may lead to pollen limitation which can threaten population persistence. Consequently, avoidance of pollen limitation is expected in outcrossing species subjected to habitually low pollinator service. The elevational decline in visitation rates on many high mountains provides an outstanding opportunity for addressing this question.

Plastic Responses Contribute to Explaining Altitudinal and Temporal Variation in Potential Flower Longevity in High Andean Rhodolirion montanum.

The tendency for flower longevity to increase with altitude is believed by many alpine ecologists to play an important role in compensating for low pollination rates at high altitudes due to cold and variable weather conditions. However, current studies documenting an altitudinal increase in flower longevity in the alpine habitat derive principally from studies on open-pollinated flowers where lower pollinator visitation rates at higher altitudes will tend to lead to flower senescence later in the life-span of a flower in comparison with lower altitudes, and thus could confound the real altitudinal pattern in a species´ potential flower longevity. In a two-year study we tested the hypothesis that a plastic effect of temperature on flower longevity could contribute to an altitudinal increase in potential flower longevity measured in pollinator-excluded flowers in high Andean Rhodolirium montanum Phil.

Dynamic sensory cues shape song structure in Drosophila.

The generation of acoustic communication signals is widespread across the animal kingdom, and males of many species, including Drosophilidae, produce patterned courtship songs to increase their chance of success with a female. For some animals, song structure can vary considerably from one rendition to the next; neural noise within pattern generating circuits is widely assumed to be the primary source of such variability, and statistical models that incorporate neural noise are successful at reproducing the full variation present in natural songs. In direct contrast, here we demonstrate that much of the pattern variability in Drosophila courtship song can be explained by taking into account the dynamic sensory experience of the male.

Temperature-driven flower longevity in a high-alpine species of Oxalis influences reproductive assurance.

How high-alpine plants confront stochastic conditions for animal pollination is a critical question. We investigated the effect of temperature on potential flower longevity (FL) measured in pollinator-excluded flowers and actual FL measured in pollinated flowers in self-incompatible Oxalis compacta and evaluated if plastically prolonged potential FL can ameliorate slow pollination under cool conditions. Pollinator-excluded and hand-pollinated flowers were experimentally warmed with open-top chambers (OTCs) on a site at 3470 m above sea level (asl).

Fast GCaMPs for improved tracking of neuronal activity.

The use of genetically encodable calcium indicator proteins to monitor neuronal activity is hampered by slow response times and a narrow Ca(2+)-sensitive range. Here we identify three performance-limiting features of GCaMP3, a popular genetically encodable calcium indicator protein. First, we find that affinity is regulated by the calmodulin domain's Ca(2+)-chelating residues.

A neuropeptide circuit that coordinates sperm transfer and copulation duration in Drosophila.

Innate behaviors are often executed in concert with accompanying physiological programs. How this coordination is achieved is poorly understood. Mating behavior and the transfer of sperm and seminal fluid (SSFT) provide a model for understanding how concerted behavioral and physiological programs are coordinated.

Patient-provider communication and reproductive health among HIV-positive women in Rio de Janeiro, Brazil.

Objective: To qualitatively assess the influence of patient-provider communication on contraceptive choice among HIV-positive women in the context of universal antiretroviral therapy (ART) access.

Methods: Focus group discussions (FGD; n=3), in-depth (IDI; n=15) and freelist interviews (FLI; n=36) were conducted with HIV-positive women aged 18-40 years recruited from public health units in Rio de Janeiro/Brazil.

Results: Of 70 participants, 49 used ART and the median time since HIV diagnosis was 6 years (range: 1-18).