Forensic application of sandpaper spray ionization mass spectrometry (SPS-MS): Direct analysis of solid pharmaceutical formulations and edible cannabis products.

In this work, we employed a new ambient ionization mass spectrometry technique, sandpaper spray mass spectrometry (SPS-MS), as an efficient tool to analyze pills and tablets of pharmaceutical formulations. The following samples were analyzed: regulators of blood pressure, cholesterol, and diabetes, and drugs for the treatment of erectile dysfunction (ED). Additionally, a hard candy of Cannabis sativa containing Δ9-tetrahydrocannabinol (Δ-THC) and its related isomer cannabidiol (CBD) was also evaluated.

Sandpaper: Efficient Medium for Analysis of Polishable Solid Samples by Ambient Mass Spectrometry.

In this paper, we employed sandpaper as an efficient medium to analyze polishable solid samples. As proof of principle, the surfaces of coffee beans were sanded with pieces of sandpaper cut into a triangular shape. The triangle was placed in front of the mass spectrometer inlet, and methanol was dropped on its surface.

Antiplasmodial activity of coumarins isolated from : and studies.

is found in the Brazilian semiarid region. This specie is little chemically and biologically studied. spp.

Assessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts.

Species of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats.

Asemeia ovata (Polygalaceae): Quantitative determination and evaluation in silico of identified substances by HPLC-DAD.

Background: In Brazil, the Asemeia genus has 19 species (12 endemic) and 2 varieties (both endemic) and some of them are found in semi-arid Bahia.

Objective: The objective of this study was to quantitatively determine identified substances by HPLC-DAD in Asemeia ovata extracts and to predict their biological activities in silico.

Method: The quantification method by HPLC-DAD has been validated according to the guidelines of the International Conference of Harmonization.

Dengue in the State of Rio Grande do Norte, Brazil, 2010-2012.

Objective: To present results of virological surveillance and epidemiological aspects of dengue in the State of Rio Grande do Norte, Brazil.

Methods: A total of 1581 cases, reported from 2010 to 2012 at various health centres in the state, were analysed by viral isolation and/or RT-PCR for viral detection and typing. To identify whether different genotypes were circulating in the state during this period, sequencing of the complete E gene for DENV (1485 bp in length) was performed directly from patient serum samples.