Publications by authors named "Diego C de Mello"
Article Synopsis
- Non-coding RNAs (ncRNAs) are now recognized as important regulators in cellular biology, especially in the context of papillary thyroid cancer, where their expression in the DLK1-DIO3 region is significantly reduced.
- The study explores how epigenetic changes, such as DNA methylation and histone modifications, might influence ncRNA expression during thyroid cancer development, employing advanced techniques like bisulfite sequencing and ChIP-qPCR for analysis.
- Findings indicate specific DNA methylation patterns linked to the downregulation of the lncRNA MEG3-DMR in tumors, and pharmacological treatments that target these epigenetic changes can restore ncRNA expression, shedding light on the complex regulation of the DLK1-DIO3
Anaplastic thyroid cancer (ATC) is a rare and lethal form of thyroid cancer that requires urgent investigation of new molecular targets involved in its aggressive biology. In this context, the overactivation of Polycomb Repressive Complex 2/EZH2, which induces chromatin compaction, is frequently observed in aggressive solid tumors, making the EZH2 methyltransferase a potential target for treatment. However, the deregulation of chromatin accessibility is yet not fully investigated in thyroid cancer.
View Article and Find Full Text PDFImportant advances on the role of genetic alterations in thyroid cancer have been achieved in the last two decades. One key reason is linked to the development of technical approaches that allowed for the mimicking of genetic alterations in vitro and in vivo and, more recently, the gene editing methodology. The CRISPR/Cas methodology has emerged as a tangible tool for editing virtually any DNA sequence in the genome.
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