Media improvement for 10 L bioreactor production of rPOXA 1B laccase by .

In this work, we statistically improved culture media for rPOXA 1B laccase production, expressed in containing pGAPZαA-- construct and assayed at 10 L bioreactor production scale (6 L effective work volume). The concentrated enzyme was evaluated for temperature and pH stability and kinetic parameter, characterized by monitoring oxidation of different ABTS [2, 20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] substrate concentrations. Plackett-Burman experimental design (PBED) implementation improved previous work results by 3.