Screening of drugs and toxic compounds with liquid chromatography-linear ion trap tandem mass spectrometry.

Background: In clinical and forensic toxicology, general unknown screening is used to detect and identify exogenous compounds. In this study, we aimed to develop a comprehensive general unknown screening method based on liquid chromatography coupled with a hybrid triple-quadrupole linear ion trap mass spectrometer.

Methods: After solid-phase extraction, separation was performed using gradient reversed-phase chromatography.