A simple fluorescent probe for selectively detecting Al and F in living cells and growing tea plants.

Aluminum (Al) and fluorine (F) ions can be easily enriched in tea plants. When they excessively accumulate in tea, they can affect the health of tea lovers. Herein, a simple, highly sensitive and selective fluorescent probe (named BHMP) for Al and F detection was developed through a one-step condensation reaction, in which benzothiazole acted as a fluorophore and acceptor and hydrazine-Schiff base as a recognition unit.

Development of a rapid and robust hydrop interaction liquid chromatography tandem mass spectrometry method for the detection of 13 endogenous amino acids as well as trimethylamine oxide in serum and tissues of the mice.

This work aimed to establish an HILIC-MS/MS method to simultaneously determine the levels of 13 endogenous amino acids and trimethylamine oxide in the biological samples from the mice. Electrospray ion source was used for the analysis of mass spectrometry. The 20 min separation was applied in a Dikma Inspire Hilic column (2.

Simultaneous two-color visualization of lipid droplets and lysosomes for cell homeostasis monitoring using a single fluorescent probe.

Lipid droplets (LDs) and lysosomes are vital organelles that play crucial roles in various physiological and pathological processes. However, simultaneous two-color visualization of these two organelles using a single probe for cell homeostasis monitoring remains a challenge due to the lack of rational design strategies. To address this issue, we have developed an aggregation-induced emission (AIE) fluorescent probe named TPE-NDI-Mor with an electron donor (D)-acceptor (A) structure, which can stain both LDs and lysosomes with high selectivity through green and red fluorescence imaging, respectively.

Liquid core fluorescent organic nanoprobes: Long-term stability and highly selective lipid droplets bio-imaging.

Lipid droplets (LDs) are important subcellular organelles that play a huge role in cell metabolism and growth. In this study, we synthesized two LDs fluorescent probes with benzothiadiazole (BTH) as electron acceptor and triphenylamine (TPA) as electron donor, which named as TPA-BTH1 and TPA-BTH2, respectively. Meanwhile, we introduced long alkyl chain to the probe as a shielding group and LDs targeting enhancement group.