[Investigation of Efflux Pump Genes in Resistant Mycobacterium tuberculosis Complex Clinical Isolates Exposed to First Line Antituberculosis Drugs and Verapamil Combination].

Tuberculosis (TB) is caused by Mycobacterium tuberculosis, still one of the most common life-threatening infectious diseases worldwide. Although drug resistance in M.tuberculosis is mainly due to spontaneous chromosomal mutations in genes encoding drug target or drug activating enzymes, the resistance cannot be explained only by these mutations.

Follow-up study of anti-SARS-CoV-2 IgG antibody response in COVID-19 patients up to 6 months after infection.

The aim of the current study was to investigate the relationship between lung involvement of SARS-CoV-2 and antibody levels of COVID-19 patients 3 and 6 months from the disease. A total of 156 participants were divided into two groups, Group 1: lung involvement (LI)-positive and Group 2: LI-negative. Biochemical parameters and anti-SARS-CoV-2 IgG antibody levels were measured.

Determination of genetic diversity of multidrug-resistant strains in Turkey using 15 locus MIRU-VNTR and spoligotyping methods.

Tuberculosis (TB) remains the leading cause of deaths from infectious disease worldwide. Nowadays, the tendency of complex (MTBC) to spread between continents due to uncontrolled migration movements shows that TB is a global health problem. The number of studies for the detection of MTBC strains' epidemiological features in areas with TB spread risk using molecular-based methods such as spoligotyping and Mycobacterial Interspersed Repetitive Unit (MIRU) Variable Number Tandem Repeats (VNTR) at the clonal level is insufficient.

[Investigation of pncA, rpsA and panD Gene Mutations Associated with Resistance in Pyrazinamide-Resistant Mycobacterium tuberculosis Isolates and Spoligotyping].

Pyrazinamide (PZA) is one of the first-line anti-tuberculous drugs used in the treatment of tuberculosis (TB). Considering the ability of PZA to shorten the treatment period from 9-12 months to six months by eliminating persistent bacilli, it appears to be an important cornerstone of TB therapy. While the main mechanism causing the PZA resistance is pncA mutations at a rate of 70-97%, it has been determined that rpsA and panD mutations can also cause resistance.

[Antibody Response After Two Doses of Inactivated SARS-CoV-2 Vaccine in Healthcare Workers with and without Previous COVID-19 Infection: A Prospective Observational Study].

In this study, we aimed to investigate the anti-S1 IgG response that occurs after inactivated severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) vaccine and the factors affecting this response in healthcare workers (HCWs) who are in the risk group for coronavirus disease 2019 (COVID-19). A total of 276 HCWs, of whom 82 previously exposed to COVID-19 infection and 194 naive who are working in Kafkas University Faculty of Medicine, Health Research and Application Center were included in this study. Anti-SARS-CoV-2 QuantiVac ELISA IgG (Euroimmun, Lubeck, Germany) kit, coated with recombinant S1 antigen including the receptor binding domain of the SARS-CoV-2 S protein was used for quantitative determination of the humoral immune response in serum samples 28 days after the first and second dose of vaccination.

[Mortality Risk Factors for Invasive Candidiasis in Pediatric Intensive Care Unit].

Invasive Candida infections are one of the most important risk factors for the increasing mortality of immunocompromised patients with comorbidities in intensive care units. In this study, it was aimed to evaluate the mortality rate and risk factors affecting mortality in patients followed up with the diagnosis of invasive candidiasis in our pediatric intensive care unit. Patients who were between the ages of 1 month and 18 years followed up in the paediatric intensive care unit with invasive candidiasis between 2014 and 2018, were included in the study.

Molecular characterization, phylogenetic and variation analyses of SARS-CoV-2 strains in Turkey.

We present the sequence and single-nucleotide polymorphism (SNP) analysis for 47 complete genomes for SARS-CoV-2 isolates on Turkish patients. The Illumina MiSeq platform was used for sequencing the libraries. The SNPs were detected by using Genome Analysis Toolkit - HaplotypeCaller v.

Prevalence of COVID-19 in 10,000 samples from 7853 patients in Eastern Turkey by positive real-time PCR.

COVID-19, caused by SARS-CoV-2, started in December 2019 and has spread across the world. We analyzed real-time PCR results of 10,000 samples from 2 April to 30 May 2020 in three neighbor cities located in the East of Turkey. The final study population was 7853 cases, after excluding screening tests.

Saliva samples for detection of SARS-CoV-2 in mildly symptomatic and asymptomatic patients.

Background: The ongoing coronavirus disease 2019 (Covid-19) pandemic has been rapidly spreading throughout the world with confirmed case numbers already exceeding 75 million. Although nasopharyngeal swabs are the most commonly utilized samples for based severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA detection, collecting these specimens requires healthcare workers and necessitates the use of personal protective equipment as it presents a nosocomial transmission risk. We aimed to assess the diagnostic value of saliva samples in mildly symptomatic and asymptomatic patients with confirmed Covid-19.

[Detection of virulence factors and antifungal susceptibilities of Fusarium strains isolated from keratitis cases].

Fusarium species have gained importance as a cause of keratitis. The pathogenicity and virulence factors of genus Fusarium remain largely unknown. Several putative virulence factors have been reported for fungal pathogens, including biofilm formation, production of proteinases and other hydrolytic enzymes.

[Serological investigation of phlebovirus exposure in blood donors from the Mediterranean Province of Mersin, Turkey].

Phleboviruses are enveloped segmented RNA viruses, capable of inducing febrile disease and/or meningoencephalitis in exposed individuals, according to the infecting strain, following transmission via arthropods. Prototype medically-important phlebovirus strains responsible for sandfly fever are sandfly fever Sicilian virus (SFSV) and sandfly fever Naples virus (SFNV), where the SFSV variant sandfly fever Cyprus virus (SFCV) is also detected in individuals with febrile disease. Toscana virus (TOSV) is unique among phleboviruses as the cause of infections involving central nervous system.

Human papillomavirus genotype distribution and E6/E7 oncogene expression in Turkish women with cervical cytological findings.

Background: Infection with certain human papillomavirus (HPV) genotypes is the most important risk factor related with cervical cancer. The objective of the present study was to investigate the prevalence of HPV infection, the distribution of HPV genotypes and HPV E6/E7 oncogene mRNA expression in Turkish women with different cervical cytological findings in Mersin province, Southern Turkey.

Materials And Methods: A total of 476 cytological samples belonging to women with normal and abnormal cervical Pap smears were enrolled in the study.