Thermostable and organic solvent-tolerant acid pectinase from FP6: purification, characterization and evaluation of its phytopigment extraction potential.

The present study discusses the purification, characterization and application of pectinase from FP6 in fruit pigment extraction. By the four-step purification involving precipitation, dialysis, ion-exchange chromatography, gel filtration chromatography, a 20.85-fold purification of the enzyme to homogeneity was achieved.