Inflammatory status hepatic enzymes and serum creatinine in HIV-, HIV+ and HIV-TB co-infected adult Central Africans.

Background And Aim: Human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome is a major public health issue in Africa. The objective of this study was to determine which of isolated HIV-infection, isolated naive pulmonary tuberculosis (PTB), or naive HIV-PTB coinfection was more harmful to inflammatory, hepatic, and renal functions.

Methods: This cross-sectional study was undertaken among ten patients with isolated HIV infection, ten patients with isolated naive HIV infection, ten patients with isolated PTB and 32 patients with HIV-PTB coinfection, with the aim of determining which group had the highest levels of oxidative stress and hepatic and renal dysfunction markers.

Hypoglycemic and antihyperglycemic effects of diethyl ether fraction isolated from the aqueous extract of the leaves of Cogniauxia podoleana Baillon in normal and alloxan-induced diabetic rats.

Cogniauxia podoleana Baillon (Cucurbitaceae family) leaves are used in Congolese traditional medicine for the treatment of diabetes mellitus. Based on an increasing number of reports on blood glucose level reduction associated with some saponins and flavonoids isolated from medicinal plants, the hypoglycemic and antihyperglycemic effects of flavonoid and saponin fractions were investigated. Saponin fractions were obtained by the fractionation of aqueous extract of leaves using benzene and ethyl acetate.

Evaluation of analgesic effect of Datura fastuosa leaves and seed extracts.

The aqueous extracts of Datura fastuosa leaves and seeds were evaluated for the analgesic effect on acetic acid-induced writhing and hot plate reaction in mice. According to the results, D. fastuosa leaves and seeds extracts at oral doses of 400 and 800 mg/kg are effective as analgesic.

Analgesic, antipyretic and anti-inflammatory effects of essential oil of Lippia multiflora.

The essential oil of Lippia multiflora Moldenke (Verbenaceae) produced by conventional hydrodistillation was analyzed and studied for its analgesic, antipyretic and anti-inflammatory activities in rats and mice. At the doses used (2, 4 and 8 ml/kg o.s.

[Psychopharmacological properties of crude extract and essential oil of Lippia multiflora].

Psychopharmacological properties of crude extract and essential oil of Lippia multiflora (Lm), a verbenacea of african traditional pharmacopea were investigated in rat using classical methods. The extract of Lm is constituted by an infusion of dried leaves. The essential oil is obtained by hydrodistillation of the dried leaves of Lm with a yield of 0.

Purification of cytoplasmic precursors of yeast mitochondrial phenylalanyl-tRNA synthetase subunits.

Two polypeptidic precursors of yeast mitochondrial phenylalanyl-tRNA synthetase subunits were purified from the cytoplasm by immunoprecipitation with an insolubilized glutaraldehyde-treated IgG fraction, followed by two chromatographies on Sephadex G-200 and on DEAE-cellulose. Methionine was found as the N-terminal residue in both precursors, which exhibited N-terminal extensions.

Mitochondrial phenylalanyl t-RNA synthetase from yeast: formation of enzyme-substrate complexes shown by heat or SH reagent inactivation.

The binding of substrates to purified mitochondrial phenylalanyl-tRNA synthetase from yeast was examined using the kinetics of heat or p-hydroxymercurybenzoate inactivation. Individually magnesium chloride and each of the substrates protect the enzyme against thermal denaturation and p-hydroxymercurybenzoate inhibition. No enzyme protection is observed with ATP alone against p-hydroxymercurybenzoate inhibition.

Biosynthesis and transport of yeast mitochondrial phenylalanyl-tRNA synthetase.

The biosynthesis of yeast mitochondrial Phe-tRNA synthetase is studied in vivo. Antibodies against the enzyme are raised in rabbits. They precipitate two proteins in the post-ribosomal supernatant of the yeast cell homogenate.

[Isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia].

Conversion of large amounts of Saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from Helix pomatia hepato-pancreatic juice. The most favourable yield in two hours incubations (88 per cent) is obtained with 20 ml cytohelicase, a chitinase and glucanase enriched extract, per 400 g of yeast cells, harvested at the end of the logarithmic growth phase and preincubated in presence of 2-mercaptoethanol.