Diagnostic value of double balloon enteroscopy for small-intestinal disease: experience from China.

Background: Diseases of the small intestine include, among others, ulceration, chronic inflammation, Meckel's diverticula, vascular deformities, and cancer.

Objective: To study the diagnostic value of double balloon enteroscopy (DBE) for small-intestinal disease in a Chinese patient cohort.

Design: DBE was performed via the mouth, anus, or both approaches to diagnose small-intestinal disease.

[The diagnostic value of double balloon endoscopy in small intestine disease].

Objective: To study the diagnostic value of double-balloon endoscopy (DBE) for small intestinal diseases.

Methods: 155 patients clinically suspicious of small intestinal diseases were studied. 110 of them were male and 45 female.

[Clinical value of monitoring CD14+ monocyte human leukocyte antigen (locus) DR levels in the early stage of sepsis].

Objective: To explore the relationship of monitoring CD14(+) monocyte human leucocyte antigen (locus) DR (HLA-DR) and the outcome in the early stage of sepsis.

Methods: Thirty-six definitely diagnosed septic patients in intensive care unit (ICU) were included. CD14(+) monocyte HLA-DR levels were detected by flow cytometer on the first day of the study, and acute physiology and chronic health evaluation II (APACHE II) scores were evaluated.

[Clinical classification of Peutz-Jeghers syndrome].

Objective: To propose the clinical classification of Peutz-Jeghers syndrome (PJS).

Methods And Results: Retrospective analysis of 52 patients with PJS admitted in Nanfang Hospital from 1980 to 2003 was conducted. Twenty-four patients were found to have family history of PJS, who had a mean age of 19 years.

[Preparation oral liposome-encapsulated recombinant Helicobacter pylori heat shock protein 60 vaccine for prevention of Hp infection].

Objective: To prepare oral liposome-encapsulated recombinant Helicobacter pylori (Hp) heat shock protein 60 (Hsp60) vaccine and investigate its effect against Hp infection in mice.

Methods: The recombinant vector PET-22(+)/Hsp60 was transformed into BL21(DE3) E.coli.

Expression of Helicobacter pylori AlpA protein and its immunogenicity.

Aim: To construct a recombinant strain which expresses adhesin AlpA of Helicobacter pylori (H pylori) and to study the immunogenicity of adhesin AlpA.

Methods: Gene Ab, which was amplified from H pylori chromosomal DNA by PCR technique, was sequenced and the biological information was analyzed, and inserted into the Nco I and Not I restriction fragments of the expression vector pET-22b(+) using T4 DNA ligase. The resulting plasmid pET-AlpA was transformed into competent E.

Pathogenicty and immune prophylaxis of cag pathogenicity island gene knockout homogenic mutants.

Aim: To clarify the role of cag pathogenicity island (cagPAI) of Helicobacter pylori (H pylori ) in the pathogenicity and immune prophylaxis of H pylori infection.

Methods: Three pairs of H pylori including 3 strains of cagPAI positive wildtype bacteria and their cagPAI knockout homogenic mutants were utilized. H pylori binding to the gastric epithelial cells was analyzed by flow cytometry assays.

Simplified purification method for Clostridium difficile toxin A.

Aim: To establish the purification method for Clostridium difficile (C. difficile) toxin A.

Methods: C.

Development of an ELISA kit using monoclonal antibody to Clostridium difficile toxin A.

Aim: To establish an ELISA kit using monoclonal antibodies against Clostridium difficile (C. difficile) toxin A.

Methods: An indirect sandwich ELISA was described using the purified rabbit monospecific antiserum as capturing antibody.

Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene.

Aim: To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA.

Methods: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain.

Construction of attenuated Salmonella typhimurium Strain expressing Helicobacter pylori conservative region of adhesin antigen and its immunogenicity.

Aim: To construct a non-resistant and attenuated Salmonella typhimurium (S. typhimurium) strain which expresses conservative region of adhesion AB of Helicobacter pylori (H pylori) and evaluate its immunogenicity.

Methods: The AB gene amplified by PCR was inserted into the expression vector pYA248 containing asd gene and through two transformations introduced into the delta Cya, delta Crp, delta Asd attenuated Salmonella typhimurium strain, constructing balanced lethal attenuated Salmonella typhimurium strains X4072 (pYA248-AB).

A novel method for preparation of tissue microarray.

Aim: To improve the technique of tissue microarray (tissue chip).

Methods: A new tissue microarraying method was invented with a common microscope installed with a special holing needle, a sampling needle, and a special box fixing paraffin blocks on the microscope slide carrier. With the movement of microscope tube and objective stage on vertical and cross dimensions respectively, the holing procedure on the recipient paraffin blocks and sampling procedure of core tissue biopsies taken from the donor blocks were performed with the refitted microscope on the same platform.

[Electron microscopic observation of primary cultured laterally spreading tumor cell line].

Objective: To observe the microscopic characteristics of laterally spreading tumor (LST) cell line in primary culture.

Methods: The cells isolated from a rectum LST specimen obtained by endoscopic mucosal resection was primary cultured, followed by observation with scanning and transmission electron microscope in comparison with the cells of adenocarcinoma and normal mucosa of the rectum.

Results: Scanning and transmission electron microscopes both revealed numerous microvilli covering the surface of the LST cells, and the cytoplasm contained large quantity of lysosomes, mitochondria and phagosomes.

Expression of Helicobacter pylori Hsp60 protein and its immunogenicity.

Aim: To express Hsp60 protein of H pylori by a constructed vector and to evaluate its immunogenicity.

Methods: Hsp60 DNA was amplified by PCR and inserted into the prokaryote expression vector pET-22b (+), which was transformed into BL21 (DE3) E.coli strain to express recombinant protein.

[Regulation of Fas expression in gastric epithelium: one of the auto-immune mechanisms of Helicobacter pylori pathogenesis].

Objective: To investigate the pathogenic mechanism of Helicobacter pylori (H.pylori)-mediated gastric epithelial cell damage.

Methods: Fas expressions in gastric epithelial cell lines and freshly isolated gastric epithelial cells with or without H.

[Effects of the antibacterial peptide cecropins from Chinese oak silkworm, Antheraea pernyi on 1, 2-dimethylhydrazine-induced colon carcinogenesis in rats].

Objective: To gain insight into the putative anticancer effect of the antibacterial peptides, cecropins, from Chinese oak silkworm, Antheraea pernyi, on the cancer cells and 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis in rats.

Methods: Growth inhibitory effect of the cecropins on normal human gastric epithelial cell line (GES-1) and human colon adenocarcinoma cell line (LS-174T) was observed using a microculture tetrazolium (MTT) colorimetric methods. Male Wistar rats were divided into 4 groups.

[In vitro evaluation of the safety and biological activity of recombinant Helicobacter pylori blood group antigen-binding adhesin].

Objective: To evaluate the safety and biological activity of recombinant Helicobacter pylori (Hp) blood group antigen- binding adhesin (rBabA ) in vitro so as to investigate the feasibility of using rBabA as a Hp vaccine.

Methods: ELISA was used to measure rBabA-specific antibody in the serum of Hp-infected patients, and the proliferation of T lymphocytes in response to rBabA was examined by MTT assay. T cell apoptosis induced by rBabA was detected by diphenylamine assay.

[Cloning and immunogenicity of conservative region of adhesin gene of Helicobacter pylori].

Objective: To construct a candidate strain of Helicobacter pylori (Hp) that expresses the proteins of the conservative region of 4 adhesins (BabA2, AlpA, AlpB, and HopZ) and study its immunogenicity.

Methods: The DNA of Hp was extracted. Primers were designed according to the C-terminal structural gene sequence (called CB) of AlpA.

[A novel method for fabrication of tissue microarray].

Background & Objective: Tissue chip (tissue microarray, TMA) is one of the most important biochip techniques, just following the gene chip and the protein chip, which is one of the most important functional genomics and proteomics research methods in the post-genomic era. However, the present TMA technology has certain shortcomings, such as lack of advanced instruments, tedious procedure, and low sampling accuracy, etc. This new method was designed to improve the technology of TMA.

[Pit pattern and endoscopic mucosal resection in diagnosis and treatment of colorectal tumors].

Objective: To explore the effective methods to diagnose and treat colorectal cancer in its early stage.

Methods: 1 205 patients were examined by colonoscopy and mucosa staining with indigocarmine. The pit patterns were observed with magnifying endoscope and stereomicroscope according to the Kudo classification.

Recombinant Helicobacter pylori catalase.

Aim: To construct a recombinant strain which highly expresses catalase of Helicobacter pylori (H. pylori) and assay the activity of H. pylori catalase.

[Construction of the non-resistant attenuated Salmonella typhimurium strain expressing Helicobacter pylori catalase].

Objective: To construct a non-resistant attenuated Salmonella typhimurium (S.typhimurium) strain capable of expressing Helicobacter pylori (Hp) catalase.

Methods: After PCR amplification, the gene fragment encoding Hp catalase was inserted into the expression vector pYA248 containing asd gene, and the recombinant vector was then introduced into the host S.

A new three-layer-funnel-shaped esophagogastric anastomosis for surgical treatment of esophageal carcinoma.

Aim: To reduce the incidence of postoperative anastomotic leak, stenosis, gastroesophageal reflux (GER) for patients with esophageal carcinoma, and to evaluate the conventional method of esophagectomy and esophagogastroplasty modified by a new three-layer-funnel-shaped (TLF) esophagogastric anastomotic suturing technique.

Methods: From January 1997 to October 1999, patients with clinical stage I and II (IIa and IIb) esophageal carcinoma, which met the enrollment criteria, were surgically treated by the new method (Group A) and by conventional operation (Group B). All the patients were followed at least for 6 months.