Effect of evaporative cooling and altitude on dairy cows milk efficiency in lowlands.

The objective of this current work was to determinate the effect of high temperatures on milk production of dairy cows in southern Slovakia in the year 2015. The hypotheses that milk production is influenced by the altitude and cooling were tested. Production data included 227,500 test-day records belonging to 34 Holstein breed herds situated in lowlands, 115 to 150 m above sea level (ASL) and kept in free-stall housing.

[A new method of preparing and some properties of high molecular weight monoamine oxidase from swine liver mitochondria].

Isolation of highly purified and highly molecular monoamine oxidase (MAO) from pig liver mitochondria have been worked out. Specific activity of isolated preparation is 2700 times higher than of original mitochondria homogenate. Enzyme solubilization by digitonin, affinity chromatography purification and ultrafiltration underlie this method.

[Amine oxidase from the bacterial strain Methanosarcina barkeri 27].

A new source of the enzyme amine oxidase (AO), namely bacteria of Methanosarcina barkery 27 strain, was discovered. The method AO isolation and purification from the bacteria was developed and the enzyme substrate and inhibitory specificity was studied. Contrary to some other microbial amine oxidases the AO investigated was shown to determine benzylamine and serotonin along with tyramine, all these substrates being deaminated at the equal rate.

Formation and properties of S-protein complex with S-peptide-containing fusion protein.

A fusion protein (FP) comprised of the RNase A S-peptide and human epidermal growth factor was shown to form a stable noncovalent catalytically active complex with the RNase A S-protein at a stoichiometric ratio 1:1 with Kdiss = 5.0 x 10(-7) M. The S-protein complex with FP exhibits the pyrimidine specificity toward substrates in both reactions catalyzed by RNase S, transesterification and hydrolysis.

[Formation and properties of S-protein complexes with S-peptide-containing hybrid polypeptides].

The fused polypeptides of human epidermal growth factor and one or two S-peptide of RNase A was shown to form stoichiometric (1:1) strong noncovalent and enzymatically active complexes with S-protein of RNase A. The dissociation constants for these complexes were found to be 5.0 x 10(-7) M and 1.