A molecular atlas of the human postmenopausal fallopian tube and ovary from single-cell RNA and ATAC sequencing.

As part of the Human Cell Atlas Initiative, our goal is to generate single-cell transcriptomics (single-cell RNA sequencing [scRNA-seq], 86,708 cells) and regulatory (single-cell assay on transposase accessible chromatin sequencing [scATAC-seq], 59,830 cells) profiles of the normal postmenopausal ovary and fallopian tube (FT). The FT contains 11 major cell types, and the ovary contains 6. The dominating cell type in the FT and ovary is the stromal cell, which expresses aging-associated genes.

Patient Perceptions Impact Progression to Third-Line Therapy for Treatment of Overactive Bladder.

Purpose: We aimed to understand the reasons patients choose to pursue third-line overactive bladder (OAB) therapy.

Materials And Methods: We conducted a mixed methods study that included patient interviews and survey data. Eligible patients were diagnosed by symptoms, had tried behavioral modifications, and OAB medications enrolled from October 2018 to August 2019.

The Effect of Symptomatic Stress Urinary Incontinence on Catheterization Rates After Intradetrusor OnabotulinumtoxinA Injections.

Objectives: To determine whether catheterization rates after intradetrusor onabotulinumtoxinA injection for nonneurogenic overactive bladder and urgency incontinence differ between women with urgency urinary incontinence only and women with urgency-predominant mixed urinary incontinence.

Methods: This was a retrospective cohort study of patients that underwent intradetrusor onabotulinumtoxinA injection of 100 U for nonneurogenic urgency urinary incontinence. The primary outcome was the difference in catheterization rates between women with urgency urinary incontinence alone compared with women with urgency-predominant mixed urinary incontinence.

Racial Distribution and Characterization of Pelvic Organ Prolapse in a Hospital-Based Subspecialty Clinic.

Objectives: Prior literature has suggested a decreased prevalence of pelvic organ prolapse (POP) in Black women. We sought to describe POP rates by race, investigate whether specific types of prolapse differ based on race, and investigate the role of uterine weight and fibroids on POP.

Methods: We conducted a retrospective cohort study of new patients seen between April 2017 and April 2019 at a tertiary urogynecology clinic.

Impact of preoperative urodynamics on women undergoing pelvic organ prolapse surgery.

Introduction And Hypothesis: Preoperative urodynamic studies (UDS) are frequently performed before pelvic organ prolapse (POP) surgery to assess urethral and bladder function. The primary goal of this study is to examine how preoperative UDS are utilized and what value these studies have in patient treatment and/or counseling.

Methods: We retrospectively reviewed patients who underwent prolapse surgery and had preoperative UDS between June 2010 and February 2015.

A viscoelastic biomechanical model of the cornea describing the effect of viscosity and elasticity on hysteresis.

Purpose: To develop a method for evaluating viscosity and elasticity of the cornea and to examine the effect that both properties have on hysteresis.

Methods: A three-component spring and dashpot model was created in Simulink in Matlab to represent the purely elastic and viscoelastic behavior of the cornea during a measurement using device called an ocular response analyzer (ORA). Values for elasticity and viscosity were varied while sinusoidal stress was applied to the model.