Publications by authors named "Dianli Zhao"

Cryptophytes are ancestral photosynthetic organisms evolved from red algae through secondary endosymbiosis. They have developed alloxanthin-chlorophyll a/c2-binding proteins (ACPs) as light-harvesting complexes (LHCs). The distinctive properties of cryptophytes contribute to efficient oxygenic photosynthesis and underscore the evolutionary relationships of red-lineage plastids.

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1,3-xylan, an important organic carbon in the ocean, is peculiar to marine algae. 1,3-xylanase-secreting bacteria and their extracellular 1,3-xylanases play pivotal roles in the degradation and biomass conversion of 1,3-xylan. However, only a few 1,3-xylanase-secreting bacteria and 1,3-xylanases have been reported.

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Exosomes are extracellular vesicles secreted by diverse cell under normal or abnormal physiological conditions, which could carry a range of bioactive molecules and play significant roles in biological processes, such as intercellular communication and immune response. In the current study, a preliminary study was performed to investigate the exosomal shuttle protein in Chlamys farreri (designated as CfesPro) and to predict the potential function of exosomes in scallop innate immunity. The serum derived exosomes (designated as CfEVs) were obtained from lipopolysaccharide (LPS)-stimulated C.

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Exosomes are 30-150 nm-sized extracellular vesicles of endocytic origin that are released into the extracellular environment and play roles in cell-cell communication. Accumulating research achievements demonstrated that exosomes could act as innate immune effectors that contribute to the host defense mechanism. To better understand the immune functions of exosomes in Crassostrea gigas against bacterial stimulation, the iTRAQ LC-MS/MS approach was applied to identifying differentially expressed proteins (DEPs) of exosomes in oyster post Staphylococcus aureus and Vibrio splendidus stimulation.

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Article Synopsis
  • The same bacterial types (OTUs) are found in both the Arctic and Antarctic oceans, supporting the idea that species are widely distributed.
  • Despite this similarity, the study reveals that the actual genetic makeup (genomes) of these bacteria is not identical across the poles, suggesting that distribution at the genomic level is more complex.
  • The research indicates that geographical isolation and the divergence time of bacteria significantly influence the genetic differences observed, providing insights into how environmental factors affect marine microbial diversity.
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Extracellular enzymes, initiating the degradation of organic macromolecules, are important functional components of marine ecosystems. Measuring seawater extracellular enzyme activity (EEA) can provide fundamental information for understanding the biogeochemical cycling of organic matter in the ocean. Here we investigate the patterns of EEA and the major factors affecting the seawater EEA of Chinese marginal seas.

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Article Synopsis
  • The study focuses on developing a cold-adapted expression system using Pseudoalteromonas to overcome limitations faced by the commonly used Escherichia coli system, particularly for thermolabile proteins.
  • Researchers created a shuttle vector and established a conjugational transfer system between E. coli and the psychrophilic strain Pseudoalteromonas sp. SM20429 to facilitate protein expression.
  • The successful expression of the cold-adapted protease, pseudoalterin, at low temperatures demonstrates that this new expression system is effective for proteins that are difficult to express in E. coli, offering a promising alternative for biotechnological applications.
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Sea ice is one of the most frigid environments for marine microbes. In contrast to other ocean ecosystems, microbes in permanent sea ice are space confined and subject to many extreme conditions, which change on a seasonal basis. How these microbial communities are regulated to survive the extreme sea ice environment is largely unknown.

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To what extent the genomes of different species belonging to one genus can be diverse and the relationship between genomic differentiation and environmental factor remain unclear for oceanic bacteria. With many new bacterial genera and species being isolated from marine environments, this question warrants attention. In this study, we sequenced all the type strains of the published species of Glaciecola, a recently defined cold-adapted genus with species from diverse marine locations, to study the genomic diversity and cold-adaptation strategy in this genus.

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A Gram-reaction-negative, aerobic, non-flagellated, rod-shaped bacterium, designated strain SM1211T, was isolated from Antarctic seawater. The isolate grew at 4-35 °C and with 0-10% (w/v) NaCl. It could produce bacteriochlorophyll a, but did not reduce nitrate to nitrite or hydrolyse DNA.

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Background: Pseudoalteromonas species are a group of marine gammaproteobacteria frequently found in deep-sea sediments, which may play important roles in deep-sea sediment ecosystem. Although genome sequence analysis of Pseudoalteromonas has revealed some specific features associated with adaptation to the extreme deep-sea environment, it is still difficult to study how Pseudoalteromonas adapt to the deep-sea environment due to the lack of a genetic manipulation system. The aim of this study is to develop a genetic system in the deep-sea sedimentary bacterium Pseudoalteromonas sp.

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Protease-producing bacteria play a vital role in degrading sedimentary organic nitrogen. However, the diversity of these bacteria and their extracellular proteases in most regions remain unknown. In this paper, the diversity of the cultivable protease-producing bacteria and of bacterial extracellular proteases in the sediments of Maxwell Bay, King George Island, Antarctica was investigated.

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Article Synopsis
  • The draft genome sequence of the Antarctic sea ice bacterium Glaciecola punicea ACAM 611(T) has been reported.
  • The genome is 3.08 megabases in size and contains a blue-light-absorbing proteorhodopsin gene.
  • This genomic information will help researchers study the metabolism and ecological roles of bacteria found in sea ice environments.
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Article Synopsis
  • Pseudoalteromonas is a marine genus with psychrophilic strains that thrive in cold environments; however, research is hindered by a lack of effective genetic systems.
  • This study developed a new heterologous expression system using a cryptic plasmid pSM429 from Arctic Pseudoalteromonas, enabling genetic manipulations.
  • Key findings included the characterization of replication initiation proteins essential for the plasmid's functionality and successful expression of antibiotic resistance and cold-adapted enzyme genes in a Pseudoalteromonas strain.
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A Gram-negative, nonmotile, aerobic and oxidase- and catalase-positive bacterium, designated D25T, was isolated from the deep-sea sediments of the southern Okinawa Trough area. Phylogenetic analyses of 16S rRNA gene sequences showed that strain D25T fell within the genus Myroides, with 99.2%, 96.

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An orange-pigmented, Gram-negative, nonmotile, strictly aerobic and oxidase- and catalase-positive bacterium (SM-A87(T)) was isolated from the deep-sea sediment of the southern Okinawa Trough area. The main fatty acids were i15 : 0, i17 : 0 3OH, i15 : 1 G, i17 : 1 omega 9c, 15 : 0, i15 : 0 3OH and summed feature 3 (comprising i-15 : 0 2OH and/or 16 : 1 omega 7c). MK-6 was the predominant respiratory quinone.

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