Extraction optimization, structure features, and bioactivities of two polysaccharides from Corydalis decumbens.

Two polysaccharides (CPS1 and CPW2) from Corydalis decumbens were obtained to develop insights into natural medical resources. Optimal extraction conditions of total sugars were researched using the method of response surface methodology, polysaccharides were purified using a combination of ethanol precipitation and anion-exchange chromatography, and structure features were analyzed by scanning electron microscopy, transmission electron microscopy, and Congo-red assay. The bioactivities were estimated in terms of antioxidant and anti-inflammatory effects.

A polysaccharide from Umbilicaria yunnana: Structural characterization and anti-inflammation effects.

A polysaccharide JUYP was isolated and purified from Umbilicaria yunnana. The detailed structure of JUYP was studied using gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), methylation-GC-MS, nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM). A homogeneous polysaccharide JUYP was obtained with the yield of 21.

Structural characterization of a novel polysaccharide from Sargassum thunbergii and its antioxidant and anti-inflammation effects.

A novel polysaccharide STSP-I was isolated and purified from Sargassum thunbergii. Its structure and bioactivity were studied using gas chromatography (GC), fourier transform infrared spectroscopy (FTIR), periodate oxidation-smith degradation, partial acid hydrolysis, methylation-GC-MS, nuclear magnetic resonance (NMR), transmission electron microscopy (TEM), radicals scavenging assays and anti-inflammatory assays. STSP-I was consisted of fucose and galactose with a molar ratio of 1.

Structure of an entangled heteropolysaccharide from Pholidota chinensis Lindl and its antioxidant and anti-cancer properties.

A major polysaccharide PCP-I was isolated and purified from Pholidota chinensis Lindl. The physicochemical and structural properties of PCP-I were studied using high-performance size-exclusion chromatography (HPSEC), gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR), periodate oxidation-smith degradation, methylation-GC-MS analysis, nuclear magnetic resonance (NMR) spectroscopy and transmission electron microscopy (TEM) analysis. PCP-I was homogeneous with molecular weight (Mw) of 249kDa and composed of xylose and fucose at a molar ratio of 2.

Quantitative Structure Activity Relationship Models for the Antioxidant Activity of Polysaccharides.

In this study, quantitative structure activity relationship (QSAR) models for the antioxidant activity of polysaccharides were developed with 50% effective concentration (EC50) as the dependent variable. To establish optimum QSAR models, multiple linear regressions (MLR), support vector machines (SVM) and artificial neural networks (ANN) were used, and 11 molecular descriptors were selected. The optimum QSAR model for predicting EC50 of DPPH-scavenging activity consisted of four major descriptors.

Structure elucidation of a non-branched and entangled heteropolysaccharide from Tremella sanguinea Peng and its antioxidant activity.

A crude polysaccharide was extracted from the edible fungi Tremella sanguinea Peng, and a polysaccharide TSP-II (31.56%) was separated and purified from the crude polysaccharide. TSP-II was a homogeneous polysaccharide by the high-performance size-exclusion chromatography (HPSEC), had a molecular weight of 356kD and consisted mainly of mannose, xylose, galactose and glucose at a molar ratio 5.

Structure elucidation of a major fucopyranose-rich heteropolysaccharide (STP-II) from Sargassum thunbergii.

A crude polysaccharide was extracted from the edible algae S. thunbergii. DEAE-Sepharose CL-6B column chromatography was used to separate and purify a major polysaccharide STP-II (63.

Extraction Optimization, Characterization and Bioactivities of a Major Polysaccharide from Sargassum thunbergii.

Sargassum thunbergii is a kind of natural edible algae. STP (S. thunbergii polysaccharides) was considered as the main bioactive compounds in S.

Structural investigation of a polysaccharide (DMB) purified from Dioscorea nipponica Makino.

A water-soluble polysaccharide was isolated from the aqueous extract of Dioscored nipponica Makino. Compositional analysis, IR analysis, methylation analysis, and NMR studies ((1)H, (13)C, (1)H(1)H COSY, HSQC, and HMBC) revealed the presence of the following repeating unit in the polysaccharide:

Optimization of total polysaccharide extraction from Dioscorea nipponica Makino using response surface methodology and uniform design.

On the base of single factor investigation, effects of extraction temperature, extraction time and ratio of water to material as well as their interactions on the yield of total polysaccharide from Dioscorea nipponica Makino were studied by uniform design and response surface methodology, respectively. The optimal process conditions were obtained by response surface methodology as follows: ratio of liquid to solid 33:1, extracting duration 134 min, and extracting temperature 95 °C, under optimized conditions, and the experimental yield 3.82% agreed closely with the predicted yield 3.

[The Elp4 subunit of human Elongator complex partially complements the growth defects of yeast ELP4 deletion strain].

In this study, we performed in vivo experiments to determine the function of human Elongator subunit Elp4 by using a yeast complementary system. Our results indicated that though human ELP4 was not able to complement the growth defects of the ELP4 deletion mutant strain to high concentration salt, it partially reduced the sensitivity of mutant strain to caffeine, high temperature and 6-AU. Gene expression analysis indicated that human ELP4 partially resumed the slow activation of the PHO5 gene caused by the deletion of yELP4 under the low phosphate concentration.