Publications by authors named "Diane Lima"

This work reports the interaction of γ-rays with MoO in several solvents to obtain non-stoichiometric (sub-oxide) MoO through a one-pot synthesis. The effect of different doses of γ-radiation (30-90 kGy) with different protic solvents (water, ,-dimethylformamide and formic acid) was investigated. Structural modifications were characterized by X-ray diffractometry and scanning electron microscopy while optical properties were investigated by UV-Vis spectroscopy.

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Campylobacteriosis is one of the most common types of bacterial gastroenteritis affecting humans, and poultry is considered a major source of the causative organism, Campylobacter spp. Broilers may arrive contaminated at slaughterhouses, and transport crates could be considered a potential source of contamination. Thus, cleaning and disinfection procedures are crucial to avoid cross-contamination among flocks.

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Viral metagenomics coupled to high-throughput sequencing has provided a powerful tool for large-scale detection of known and unknown viruses associated to distinct hosts and environments. Using this approach, known and novel viruses have been characterized from sylvatic and commercial avian hosts, increasing our understanding of the viral diversity in these species. In the present work we applied an exploratory viral metagenomics on organs (spleen, liver and bursa of Fabricious) of Pekin ducks from Southern Brazil.

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Article Synopsis
  • Porcine circovirus 3 (PCV3) has been found in swine globally since 2016, appearing in both healthy and diseased animals.
  • A study looked into whether PCV3 in the blood of sows was linked to stillbirths, analyzing 89 serum samples from newly farrowed sows.
  • The results showed that while PCV3 was detected in many samples, there was no significant difference in infection rates or viral loads between sows with and without stillbirths, indicating no direct connection.
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In the present study, the complete nucleotide sequence of porcine circovirus 3 (PCV3) recovered from wild boars lymph nodes is described. The full genome was named PCV3-wb/Br/RS and comprises 2,000 nucleotides with two open reading frames (ORFs) with a stem-loop motif in intergenic region. The ORFs are oriented in opposite directions and encode the putative capsid (Cap) and replicase (Rep) proteins.

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Malabsorption syndrome (MAS) is an economically important disease of young, commercially reared broilers, characterized by growth retardation, defective feather development and diarrheic faeces. Several viruses have been tentatively associated to such syndrome. Here, in order to examine potential associations between enteric viruses and MAS, the faecal viromes of 70 stool samples collected from diseased (n = 35) and healthy (n = 35) chickens from seven flocks were characterized and compared.

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Salmonella Enteritidis and Salmonella Typhimurium are among the most prevalent serotypes isolated from salmonellosis outbreaks and poultry. Salmonella spp. have the capacity to form biofilms on several surfaces, which can favour survival in hostile environments, such as slaughterhouses.

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This study is focused on the identification of the faecal virome of healthy chickens raised in high-density, export-driven poultry farms in Brazil. Following high-throughput sequencing, a total of 7743 de novo-assembled contigs were constructed and compared with known nucleotide/amino acid sequences from the GenBank database. Analyses with blastx revealed that 279 contigs (4 %) were related to sequences of eukaryotic viruses.

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Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus. To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), isolated from a water buffalo (Bubalus bubalis) in 1972 in Australia.

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Article Synopsis
  • Chicken parvovirus (ChPV) might be linked to a sickness called malabsorption syndrome (MAS) in chickens.
  • Scientists tested 68 sick chickens and 59 healthy ones to see if ChPV was present.
  • They found that sick chickens had way more ChPV in their bodies than the healthy ones, suggesting that higher amounts of the virus could cause the sickness.
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A novel bovine parvovirus 2 (BPV2) genotype comprising 5394 nt was identified by next generation sequencing from sera of healthy cattle at different age groups farmed in the state of Rio Grande do Sul, Brazil. The genome organization of new BPV2 genotype retains the two ORFs typical of members of the Parvovirinae with 86.4 % of overall nucleotide sequence identities in comparison to other members of the subfamily.

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