A cationic peptide consists of ornithine and histidine repeats augments gene transfer in dendritic cells.

Condensing the plasmid with high molecular weight cationic polymers such as poly-L-lysine (PLL) and poly-L-ornithine (PLO) can enhance antigen-specific immunity generated from genetic vaccination with naked DNA encoding antigens. While these high molecular weight polymers are clearly effective in transfection experiments, clinical applications are limited by their physical heterogeneity and toxicity. Three chemically defined low molecular weight cationic peptides, K(16), K(10)H(6), and O(10)H(6), were examined in the context of DNA binding, toxicity, and efficiency of gene transfer in dendritic cells (DC).