Publications by authors named "Diana Neri"

Salmonellosis is currently the second most common zoonosis in European Union but in the 6-years periods, between 2012 and 2017, there has been a significant decrease trend in the yearly number of infections caused by Salmonella. In Italy, S. Typhimurium and monophasic S.

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Campylobacteriosis is the most commonly reported gastrointestinal disease in humans. is the main cause of the infection, and bacterial colonization in broiler chickens is widespread and difficult to prevent, leading to high risk of occurrence in broiler meat. Phage therapy represents an alternative strategy to control in poultry.

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Article Synopsis
  • An outbreak of campylobacteriosis in Pescara, Italy, affected students and staff from several kindergartens and primary schools in May-June 2018, leading to an epidemiological and microbiological investigation to identify its cause.
  • A case-control study conducted with 176 cases and 62 controls found that the highest infection rate occurred on May 29, primarily linked to 'caciotta' cheese consumed during school lunches.
  • Genomic analysis confirmed that the strains isolated from infected individuals and the cheese matched, suggesting that the cheese was the source of the outbreak, likely due to a pasteurization failure.
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In this study, thirteen batches of broiler chicken from an integrated Italian poultry company were investigated for the detection of Listeria monocytogenes. The prevalence was evaluated in faeces samples at farm level and after transport, caecal contents and carcass neck skin from 2 slaughterhouses (M1 and M2), for a total of 2080 samples, throughout a 27-month period. No positive results were recorded in faeces, while the overall prevalence of contamination in carcass neck skin was 26.

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Pecorino di Farindola is a typical cheese produced in the area surrounding the village of Farindola, located in the Abruzzo Region (central Italy), unique among Italian cheese because only raw ewe milk and pig rennet are used for its production. In the literature it is well documented that raw milk is able to support the growth of pathogenic microorganisms such as Listeria monocytogenes. Predictive microbiology can be useful in order to predict growth-death kinetics of pathogenic bacteria, on the basis of known environmental conditions.

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A research was carried out in Italy with the aim of assessing Campylobacter contamination in broilers from breeding to slaughter, of defining the genetic diversity of isolates and their antibiotic resistance. Sampling was carried out in a slaughterhouse, and in farms representative of the most common broiler production in Italy. At farm, the 78.

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Article Synopsis
  • Swine are significant carriers of bacteria associated with foodborne diseases, primarily contaminating carcasses during slaughter, with a higher contamination rate found on carcasses (50.4%) compared to feces (32.9%).
  • The study assessed the antimicrobial resistance (AMR) patterns and genetic diversity of bacterial strains isolated from swine, revealing 25 distinct PFGE types and 51 sequence types, with ST-854 being the most prevalent.
  • High levels of resistance were found against antibiotics like quinolones, with many strains showing multi-resistance; a notable connection was observed between the genetic profiles of swine and human isolates, particularly those belonging to the ST-828 clonal complex.
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Campylobacter species are common foodborne pathogens associated with cases of human gastroenteritis worldwide. A detailed understanding of the prevalence, contamination levels and molecular characteristics of Campylobacter spp. in cattle and chicken, which are likely the most important sources of human contamination, is imperative.

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Campylobacter jejuni, a common foodborne zoonotic pathogen, causes gastroenteritis worldwide and is increasingly resistant to antibiotics. We aimed to investigate the antimicrobial resistance (AMR) genotypes of C. jejuni isolated from humans, poultry and birds from wild and urban Italian habitats to identify correlations between phenotypic and genotypic AMR in the isolates.

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Purpose: From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidisciplinary network.

Methodology: Epidemiological and microbiological surveillance was conducted to confirm the outbreak and to detect the food vehicle of infection.

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Traditional Italian pork products can be consumed after variable drying periods, where the temporal decrease of water activity spans from optimal to inactivating values. This makes it necessary to A) consider the bias factor when applying culture-medium-based predictive models to sausage; B) apply the dynamic version (described by differential equations) of those models; C) combine growth and death models in a continuous way, including the highly uncertain growth/no growth range separating the two regions. This paper tests the applicability of published predictive models on the responses of Listeria monocytogenes and Yersinia enterocolitica to dynamic conditions in traditional Italian pork sausage, where the environment changes from growth-supporting to inhibitory conditions, so the growth and death models need to be combined.

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Pork meat products consumed raw or after a short period of fermentation can be considered at risk for food safety. Sausages (fresh sausage made from pork meat) are produced in several Italian regions, with variation in ingredients. In some Italian Regions, including Abruzzo, these products are frequently consumed raw or undercooked, after a variable period of fermentation.

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