AI-Assisted Real-Time Immunoassay Improves Clinical Sensitivity and Specificity.

Real-time biosensing systems can interrogate the association between the analyte and the biorecognition element across time. Typically, the resulting data are preprocessed to offer valuable bioanalytical information obtained at a single optimal point of such a real-time response; for instance, a diagnosis of certain medical conditions can be established depending on a biomarker (analyte) concentration measured at an optimal time, that is, a threshold. Exploiting this conventional approach, we previously developed a nanophotonic immunoassay for bacterial vaginosis diagnosis exhibiting a clinical sensitivity and specificity of ca.

Measurement of Protein Kinetics Using a Liquid Phase-Based Biosensing Platform.

Macromolecular association is crucial to many fields in biomedical sciences, including drug development, gene editing, and diagnostics. In particular, protein-protein association and dissociation rate constants are typically determined using surface plasmon resonance systems, which require costly instrumentation and cumbersome procedures (e.g.

Facile Determination of COVID-19 Seroconversion via Nonradiative Energy Transfer.

Serological tests are crucial in a pandemic scenario, since they are a valuable tool to spot those citizens with potential immunity, specific regions with herd immunity or particular at-risk populations, as well as acquired immunity after vaccination. Hence, high-throughput, fast, cost-effective, and straightforward technologies facilitating interrogation of COVID-19 seroconversion are an existing need. Herein, we developed an innovative assay for the determination of COVID-19 seroconversion.

Microwell plates coated with graphene oxide enable advantageous real-time immunosensing platform.

Immunoassays are fundamental analytical tools in molecular diagnostics, therapy monitoring and drug discovery. Nevertheless, they often take around 6 h and require cumbersome procedures. We introduce a breakthrough in immunosensing based on the photoluminescence quenching capabilities of graphene oxide (GO) and the versatile format offered by the famous microwell plates.

Real-Time Photoluminescent Biosensing Based on Graphene Oxide-Coated Microplates: A Rapid Pathogen Detection Platform.

Pathogenic bacterial contamination is a major threat to safety, human health, and ecosystems. Herein, we report an advantageous single-step, wash-free, and real-time bacterial detection platform operating with a single antibody. was detected as a model analyte.