Trends in antimicrobial resistance of species in Peru, 2011-2020.

Objective: To describe the frequency of antimicrobial resistance rates and spatial-temporal distribution of species from the last 10 years in Peru.

Methods: A cross-sectional descriptive study was carried out. A total of 1668 strains, remitted as part of the national enteric pathogen surveillance from 2011 to 2020, were analysed.

Evaluation of the SMARTCHEK Genesystem RT-qPCR assay for the detection of SARS-CoV-2 in clinical samples.

Background: The COVID-19 pandemic remains the main public health problem, due to the quick and easy dissemination of the causal agent, SARS-CoV-2 virus, around the world. Since the beginning of the pandemic, an opportune laboratory diagnosis has been critical to respond this emergency, and RT-qPCR has been used as reference molecular tests for detection of SARS-CoV-2.

Methods: In this study, we performed the evaluation of a RT-qPCR SMARTCHEK platform (SMARTCHEK, Genesystem) for SARS-CoV-2 detection based on the amplification of RdRp and N gene markers.

Genomic Analysis and Antimicrobial Resistance of and in Peru.

is the leading cause of human bacterial gastroenteritis worldwide and has a major impact on global public health. Whole Genome Sequencing (WGS) is a powerful tool applied in the study of foodborne pathogens. The objective of the present study was to apply WGS to determine the genetic diversity, virulence factors and determinants of antimicrobial resistance of the populations of and in Peru.

Evaluation of reverse transcription-loop-mediated isothermal amplification for rapid detection of SARS-CoV-2.

The main strategy for response and control of COVID-19 demands the use of rapid, accurate diagnostic tests aimed at the first point of health care. During the emergency, an increase in asymptomatic and symptomatic cases results in a great demand for molecular tests, which is promoting the development and application of rapid diagnostic technologies. In this study, we describe the development and evaluation of RT-LAMP to detect SARS-CoV-2 based on three genes (ORF1ab, M and N genes) in monoplex and triplex format.

Molecular Characterization of Fluoroquinolone-Resistant .

The presence of amino acid changes in GyrA, GyrB, ParC, ParE, and in a proposed chromosomal chloramphenicol acetyl transferase (CAT), as well as mutations at , were established by PCR and sequencing in 38 clinical isolates from four different areas in Peru. Eighteen out of 24 (75%) isolates showing ciprofloxacin resistance for both disk-diffusion and e-test presented amino acid substitutions in GyrA (GC, six isolates, AV, 1 isolate) GyrB (SF, 10 isolates) or both (GyrA DN and GyrB SF, one isolate). Two out of 14 susceptible isolates presented amino acid substitutions at GyrB (SF) or a double substitution GyrA DN and GyrB SF.

Environmental and climatic impact on the infection and mortality of SARS-CoV-2 in Peru.

Objectives: The role of the environment and climate in the transmission and case fatality rates of SARS-CoV-2 is still being investigated a year into the pandemic. Elevation and air quality are believed to be significant factors in the development of the pandemic, but the influence of additional environmental factors remains unclear.

Methods: We explored the relationship between the cumulative number of infections and mortality cases with climate (temperature, precipitation, solar radiation, water vapor pressure, wind), environmental data (elevation, normalized difference vegetation index or NDVI, particulate matter at 2.

[Multidrug resistance of Salmonella infantis in Peru: a study through next generation sequencing].

Objectives.: To describe the phenotypic and genotypic patterns of the antimicrobial resistance of Salmonella Infantis in Peru.

Materials And Methods.

[Antimicrobial resistance of Bartonella bacilliformis strains from regions endemic to bartonellosis in Peru].

Objectives: To evaluate in vitro antimicrobial susceptibility to chloramphenicol (CHL) and ciprofloxacin (CIP) in strains of Bartonella bacilliformis from areas that are endemic to Bartonellosis in Peru, through three laboratory methods.

Materials And Methods: Antimicrobial susceptibility to CHL and CIP from 100 strains of Bartonella bacilliformis isolated in patients from the regions of Ancash, Cusco, Cajamarca, Lima and La Libertad were evaluated. Strains were evaluated by: disk diffusion, E-test and agar dilution.