The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages.

The aim of the study is to investigate the changes of serum leptin and kisspeptin levels in children and adolescents with different pubertal stages and nutritional states. A total of 647 Chinese children and adolescents were recruited, and serum estradiol, testosterone, pituitary gonadotropins, leptin, and kisspeptin levels were measured. The results showed that serum leptin levels of boys in T2 stage were the highest among the five stages, while they showed a gradual increase from T1 to T5 stage in girls and reached the highest in T5 stage ( < 0.

Serum Levels of the Adipokine Zinc- α 2-glycoprotein Are Decreased in Patients with Hypertension.

Objective. Zinc-α2-glycoprotein (ZAG) has recently been proposed as a new adipokine involved in body weight regulation. The purpose of this study is to investigate serum levels of ZAG in patients with hypertension and its association with related characteristics.

[Construction of Zinc-alpha2-glycoprotein expression plasmid and its expression in 3t3-l1 preadipocytes].

Objective: To construct mouse Zinc-alpha2-glycoprotein (mZAG) eucaryotic expression plasmid and identify its expression in 3T3-L1 preadipocytes.

Methods: The total RNA from mouse liver tissue was extracted. The reverse-transcript(RT)-PCR method was used to amplify the complete domain sequence of mZAG, and the confirmed PCR products was inserted into expression plasmid by DNA ligation.

[Effect of bodyweight on the onset of puberty of female children and adolescents].

Objective: To investigate the possible effects and roles of bodyweight on the puberty onset in adolescent girls.

Methods: Totally 288 Chinese female children and adolescent girls aged 5 to 16 were followed up yearly for four consecutive years. The height, bodyweight, fat percentage, sexual characteristics, and the serum levels of leptin and insulin-like growth factor-1 (IGF-1) were studied to analyze the influential factors of puberty onset and age of menarche.

[Puberty development of the healthy adolescent girls of Daqing city, Heilongjiang province].

Objective: To investigate the process of puberty development of healthy adolescent girls in Northern China.

Methods: 288 adolescent girls of Daqing city, Heilongjiang province, aged 5 to 16, were studied and followed up yearly for four years. The height, weight, fat percentage, second sex characteristics, and the blood levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E(2)) were examined.

[Mechanism of interleukin-1beta increasing growth hormone expression in rat pituitary GH3 cells].

Objective: To investigate the effect(s) of interleukin-1beta (IL-1beta) on the activity of human growth hormone (hGH) gene promoter in rat pituitary GH3 cells and the molecular mechanism.

Methods: The method of luciferase reporter gene was used. We firstly established stable GH3 cell line which contains hGH gene promoter -484-30 bp and luciferase reporter gene.

[The Effect of Cytokines on Expression of Growth Hormone Gene in Rat Pituitary GH(3) Cells].

To study the effect of interleukin-11(IL-11), ciliary neurotropic factor (CNTF) and transforming growth factor-beta (TGF-beta) on the hGH gene promoter activity in rat pituitary GH(3) cells and the interaction with pituitary-specific transcription factor Pit-1, firstly the stable transformed GH(3) cell line which contained hGH gene promoter 484-30 bp and luciferase reporter gene was established, then the concentration of GH in the medium and lysate of GH(3) cells and luciferase activities in GH(3) cells were measured, after treating these cells with the above cytokines, the effects of cytokines on secretion and synthesis of GH, and the promoter activity of the hGH gene were observed. The results of our experiments showed that IL-11(20 nmol/L), CNTF(10 nmol/L) and TGF-beta(5 nmol/L) regulated secretion and synthesis of GH, and the luciferase expression in stable-transformed GH(3) cells. IL-11 and CNTF had a stimulatory effect, whereas TGF-beta had an inhibitory one.