Quality control and quality assurance increasingly are under evaluation. The number of procedures required to guarantee the medical reliability of the data generated by clinical laboratories has grown steadily with little or no signs of abating. Laboratorians would do well to anticipate the cost/benefit questions that appear inevitable.
View Article and Find Full Text PDFThe genus Galago provides an unique opportunity to study the relation between layers of the lateral geniculate body and classes of retinal ganglion cells. In the present experiments HRP was restricted to individual layers of the lateral geniculate body with the following results: After injections of the magnocellular layers, layers 1 and 2, labeled retinal ganglion cells ranged in size from 8 to 20 micrometers. After injections of the parvocellular layers, layers 3 and 6, labeled retinal ganglion cells ranged in size from 6 to 12 micrometers.
View Article and Find Full Text PDFGentamicin is a nephrotoxic agent known to damage the proximal tubule,--a site of low molecular weight (LMW) protein reabsorption and catabolism. The effect of gentamicin was investigated on three LMW proteins--amylase, light chains, and beta 2 microglobulin--and the effects were correlated on the latter to renal function as determined by creatinine clearance (GFR). The renal excretion of beta 2 microglobulin (beta 2M) was studied in 18 patients receiving gentamicin and eight control patients.
View Article and Find Full Text PDFAcute febrile neutrophilic dermatosis, otherwise known as Sweet syndrome, usually occurs in women after prior respiratory infections. The first case of Sweet syndrome with involvement of the female genital tract is reported here. The woman recovered completely, and no evidence of other underlying disease has appeared.
View Article and Find Full Text PDFWe have studied the projections from the superior colliculus and the neocortex to the pulvinar nucleus in Galago senegalensis by using the retrograde transport of horseradish peroxidase (HRP). Injections of various parts of the pulvinar complex, both the inferior and superior divisions, both the tectorecipient zone and the nontectorecipient zone as defined by Glendenning et. al.
View Article and Find Full Text PDFThe practice of pre-admission hospital laboratory testing has been criticized as inefficient and cost ineffective. Laboratory screening is frequently condemned without regard for the possibility that a particular screening protocol may be poorly conceived and inadequate. To increase the specificity of our screening procedure to answer some questions raised by results of total enzyme and protein results, we have adapted the Nerenberg "sandwich" technique for multi-sample, simultaneous electrophoresis of sera for proteins, lactic dehydrogenase, and alkaline phosphatase.
View Article and Find Full Text PDFA 41,000-dalton phosphoprotein in crude synaptosomal membrane fractions is characterized by its unique divalent and monovalent cation regulation. It is identified by two-dimensional gel electrophoresis as the phosphoprotein whose phosphorylation is enhanced by repetitive electrical stimulation of hippocampal brain slices. After sucrose-gradient ultracentrifugation, this phosphoprotein is found in the mitochondrial subfraction.
View Article and Find Full Text PDFRetinal ganglion cells were labeled with HRP after injecting single layers of GL or single strata within the stratum griseum superficiale (SGS). Only small cells were labeled after injecting small cell C layers and upper SGS. Only large cells were labeled after injecting lower SGS.
View Article and Find Full Text PDFThis inquiry began with the discovery that just two layers of the lateral geniculate nucleus (GL) of Galago contain large amounts of acetylcholinesterase (AChE). These two layers (layers 3 and 6) are similar in cell size and Nissl-staining characteristics and project to the same layer in the striate cortex. To find out whether the pattern of staining is unique in the Galago, we examined the distribution of AChE in the lateral geniculate nucleus of the owl monkey, Aotus trivirgatus.
View Article and Find Full Text PDFWhipple's disease, a multisystem chronic granulomatous disease treatable by antibiotics, usually presents clinically with gastrointestinal or joint symptoms. Usually, the diagnosis is substantiated by small intestinal biopsy. This shows diastase-resistant periodic-acid-Schiff-(PAS)-positive inclusions in the cytoplasm of macrophages within the lamina propria.
View Article and Find Full Text PDFIn the first series of experiments, small amounts of HRP were injected into areas 17, 18, and 19 and each of the cytoarchitectonic areas of the temporal lobe. The resulting distributions of labeled cells fell into a number of distinctive classes. For example, after injecting the temporal anterior area (Ta), the labeled cells occupied a band on the ventral border of the inferior division of the pulvinar complex; after injecting the temporal ventral area (Tv), the labeled cells were concentrated in the medial extremity of the superior division.
View Article and Find Full Text PDFBiochim Biophys Acta
August 1980
Membrane protein phosphorylation may be a general regulatory mechanism mediating the response of cells to exogenous metabolic and physical signals. We have determined that the membrane-bound acetylcholine receptor is the major substrate phosphorylated in situ by a nearby membrane protein kinase. Moreover, these same membranes also contain phosphoprotein phosphatase activity which dephosphorylates the membrane-bound receptor.
View Article and Find Full Text PDFQuality Control is a way of life for today's clinical laboratory and the direct responsibility of its director. While individual laboratory sections require quality control procedures of ever-growing complexity, there also is need for an overview and the statement of policies that concern the laboratory as a whole. In the August 1977 issue of Pathologist (I & A Center, pp.
View Article and Find Full Text PDFA method is presented for simultaneously determining diazepam and chlordiazepoxide along with their respective major active serum metabolites N-desmethyldiazepam, and N-desmethylchlordiazepoxide and demoxepam. The drugs are extracted from one ml of buffered serum using chloroform containing 5-(p-methylphenyl)-5-phenylhydantoin as an internal standard. The elution is accomplished using a reversed-phase column with a mobile phase consisting of an acetonitrile/methanol/acetate buffer pH 5.
View Article and Find Full Text PDFJ Supramol Struct
July 1981
We have found that the acetylcholine receptor (AChR) of Torpedo californica is phosphorylated and dephosphorylated in situ by a membrane-bound protein kinase and phosphatase [1]. There is increasing evidence that other neurotransmitters [2], light [3-6], polypeptide hormones [7], and growth factors [8-12] also may act by regulating the level of phosphorylation of membrane proteins. These observations suggest that membrane protein phosphorylation may be a general regulatory mechanism affecting the response of cells to exogenous metabolic and physical signals.
View Article and Find Full Text PDFWe have examined the origin of the subcortical projections to the superficial layers of the striate cortex in Tupaia glis and Galago senegalensis by using the retrograde transport of HRP. Crystals of HRP were laid directly on the moist pial surface of the cortex which had been gently pricked with a small glass pipette. The diffusion of HRP was limited to layers I and II by restricting the length of time that the HRP was in contact with the surface.
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