[Trisomy of chromosome 8 in Ph-negative cells of the bone marrow in patients with chronic myeloid leukemia treated with inhibitors of BCR-ABL tyrosine kinases].

Aim: To analyse clinical implications of chromosome 8 trisomy in Ph-negative cells of the bone marrow in patients with chronic myeloid leukemia (CML) treated with inhibitors of tyrosinkinases (ITK).

Material And Methods: A total of 386 patients with CML (chronic phase--288, acceleration phase--77) received imatinib (400-800 mg/day). Because of resistance and/or intolerance some patients were switched to ITK II (nilotinib, dasatinib, bozutinib).

[Hairy cell leukemia in young patients].

Aim: To characterize clinical symptoms, course, immediate and long-term treatment results in young patients with hair cell leukemia (HCL).

Material And Methods: The data on 41 HCL patients were analysed. The diagnosis was made by standard diagnostic protocol for HCL detection.

[Principles and potentialities of the standardization of morphocytochemical diagnosis of acute leukemias].

Under the present conditions, the competitive capacity of a health care facility is provided by the high level and timeliness of diagnosis of disease. The diagnosis of the types of acute leukemia (AL) may be accomplished immunologically, by using a 33-marker panel and without consideration of the morphocytochemical parameters of blast cells. But such an approach complicates and prolongs the examination of patients with AL.

[Clinical significance of erythrocyte ferritin in refractory anemia and chronic myeloid leukemia].

Aim: To find out if the RBC ferritin elevation can serve as an additional criterion of inefficient erythropoiesis during progression of chronic myeloid leukemia (CML) and in various types of refractory anemia.

Material And Methods: The study group consisted of 56 MDS patients and 73 patients at various stages of CML. 20 healthy donors and 105 patients with verified inefficient erythropoiesis (20--with B12 deficiency before and after the treatment, 85--with beta-thalassemia) were the controls.

[Effectiveness of cyclosporin A in the treatment of adult patients with aplastic anemia].

Aim: To evaluate the efficacy of cyclosporin A (CyA) at different stages of immunosuppressive therapy (IST) in patients with aplastic anemia (AA).

Materials And Methods: The efficacy of CyA was studied in 56 patients with AA. The agent was orally given in an initial dose of 10 mg/kg as solution or capsules.

[Quantitative morphology of periferal erythrocytes and bone marrow erythrokaryocytes in acute leukemia and hemopoietic depressions].

Morphology of peripheral blood erythrocytes was studied in patients with acute leukemia and aplastic anemia by a Russian cytoanalyzer Mekos-C. Twenty-eight patients with acute myeloblastic leukemia, 15 with acute lymphoblastic leukemia, and 11 with aplastic anemia were examined. Erythrocytes (n = 500) were examined in fixed non-stained blood smears.

[Physiological (programmed) cell death in hemopoiesis].

According to current concepts, pluripotent cells proliferate and differentiate into "committed" precursors. These committed precursors divide, mature, and give rise to red cells, granulocytes, monocytes, and platelets of the blood. The life span of mature circulating cells being short, and their populations in the blood very stable, a constant and strict regulation of hematopoiesis is needed.

[A "primitive" variant of the blast crisis in chronic myeloleukemia].

While immunotyping blast cells from 45 patients with CML blast crisis, we detected 5 cases with immunologically primitive blast cells. The immunological phenotype of these cells corresponded to that of primitive stem cells which are characterized by expression of CD34 and HLA-DR antigens in the absence of other immunological markers. We suggest that blast cells from these patients may undergo differentiation similar to that of primitive stem cells that implies the existence of a new immunological variant of CML blast crisis, a primitive variant.

[Possible relationship between the cytologic characteristics of mouse leukemia P-388 cells and their sensitivity to rubomycin].

In search for markers of tumor cell resistance to cytostatic drugs, cytologic characteristics were compared of resistant and sensitive to rubomycin variants of mouse P-388 leukemia. Morphocytochemical, morphometrical and cytogenetic investigations were performed as well as measurements of intracellular pH. As shown by the fluorescent test, pH levels in rubomycin-sensitive and resistant subpopulations differed.

[Morphocytochemical characteristics of hemopoietic cells in sideroblastic anemia].

The findings of morphocytochemical and cytogenetic examinations of hemopoietic cells in 8 patients with sideroblastic anemia are analyzed. The authors consider that the final diagnosis of sideroblastic anemia can be made if the count of ring-shaped sideroblasts surpasses 30%. The leukemic nature of this condition is discussed.

[Beta-glucuronidase activity and the cellular ultrastructure of the lymph nodes in malignant non-Hodgkin lymphomas].

Lymphoid elements from lymph nodes of malignant non-Hodgkin's lymphomas were studied by the methods of beta-glucuronidase cytochemistry, scanning, and transmission electron microscopy. The number of lysosomes, more prominent in nonmature variants, was found to decrease.