[Detection of antibodies to M. leprae in saliva of lepric patients].

Saliva of 116 lepric patients were scanned for antibodies in ELISA (with M. leprae cultivated in vitro used as antigen) in order to work out an invasion-free diagnostic tool for lepra. The ELISA findings for "saliva-serum" pairs from same patients showed an increased level of antibodies both in serum and saliva in 39.

[Leprous neuropathies].

The damage to the peripheral nervous system (PNS) is a marker of Micobacterium leprae (M. leprae) infection that develops as a result of the M. leprae invasion to the Schwann cells.

[Electron microscopy of Mycobacterium leprae passed in laboratory animals].

The ultrastructure of M. leprae was studied in the process of its intraplantar passage from man to mice (passages 1-8). Changes in the morphology of M.

[Comparative characterization of the antigenic composition of M. leprae and M. lufu].

The antigenic structure of M. leprae and M. lufu was comparatively studied for the first time.

[The comparative cultural-biochemical and immunochemical identification of laboratory strains of cultured mycobacteria from leprous patient lesions and from soil].

The paper presents the results of studied of cultural biochemical and immunochemical properties of the cultured mycobacteria isolated from patients with leprous lesions of (M.01, M.011) and the soil (M.

[The electron immunochemical detection of mycobacterial antigens in the granulomas of leprosy patients].

M.leprae antigens (Ag) in skin granuloma macrophages from 10 untreated patients with active lepromatous leprosy were studied with the use of electron microscopic immunochemical methods. Peroxidase-conjugated antirabbit antibodies to M.

[A microtitration variant of the latex agglutination reaction in the diagnosis of leprosy].

The authors describe the preparation of antigenic diagnostic agent based on stained polyacrolein latex particles conjugated with beta-(3-aminopropyl) 3, 6-dimethylglucopiranoside (DMG), a synthetic analog of PSL = 1 from M. leprae (L-PMG), and its use in the microtitration variant of latex agglutination for the serological diagnosis of lepra. The test was performed in polystyrene plates.

[The isolation of monoclonal antibodies to strains of Mycobacterium leprae passed through laboratory animals].

Hybridoma synthetizing monoclonal antibodies (mAB) IIIE4 has been obtained in hybridization of myeloma P3-X63-Ag8. 653 cells and splenocytes from BALB/c mice immunized with M. leprae passaged on rats.

[Diagnostic test systems based on the immunoenzyme method in leprosy].

Diagnostic test systems for the detection of IgG and IgM to Mycobacterium leprae in the blood sera of leprosy patients and armadillos experimentally infected with M. leprae have been developed on the basis of the indirect immunoperoxidase assay. The possibility has been shown of prognosing the activity of the leprotic process in leprosy patients and the results of the experimental infection of armadillos by the dynamic increase of antibody reactions with the development of the infection.