[An interactive volume rendering algorithm for laser scanning confocal microscope data].

Objective: To satisfy different observational demands, a real-time interactive volume rendering for laser scanning confocal microscope (LSCM) image was realized on the PC.

Method: An interactive transfer function was proposed through analyzing optical properties of LSCM image, so that the users can enroll in exploring the LSCM data set by altering rendering parameters. The texture mapping based on visualization method is adopted to realize real-time 3D reconstruction.

Nitric oxide spatial distribution in single cultured hippocampus neurons: investigation by projection of reconstructed 3-D image and visualization technique.

Recent studies have revealed a non-homogeneous distribution of nitric oxide synthase (NOS) in neurons. However, it is not yet clear whether the intracellular distribution of NOS represents the intracellular nitric oxide (NO) distribution. In the present study, software developed in our laboratory was applied to the reconstructed image obtained from confocal slice images in order to project the 3-D reconstructed images in any direction and to cut the neuron in different sections.

The influence of oxygen-glucose deprivation on nitric oxide and intracellular Ca(2+) in cultured hippocampal neurons.

Nitric oxide (NO) was speculated to play an important role in the pathophysiology of cerebral ischemia. In this study, the effect of oxygen-glucose deprivation (OGD) on the cellular production of NO was investigated in cultured hippocampal neurons. Intracellular Ca(2+) was also detected as its closely relationship with NO.