pCold-assisted expression of a thermostable xylanase from : cloning, expression and characterization.

Unlabelled: The biotechnological application of bacterial xylanases requires a high thermostability, a catalytically active state for a broad pH range. The (MTCC 1270) A gene was amplified and cloned into the pCold vector and was expressed in to evaluate the expressed proteins' thermostability The pCold, compared to other similar vectors, has unique properties-including pH and temperature tolerance due to the presence of the cspA promoter. The recombinant A-pCold (rxynApC) showed the expression of A gene with a molecular weight of ~ 27 kDa, confirmed on SDS-PAGE.

Linkers: A synergistic way for the synthesis of chimeric proteins.

In the cell, the protein domains are attached with the short oligopeptide, commonly known as linker peptide. Besides bridging, the linker assists in the domain-domain interaction and protein folding into the peculiar conformations. Linkers allow or control the movement of protein domains in the dynamic cellular environment.