Fabrication of a microvesicles-incorporated fibrous membrane for controlled delivery applications in tissue engineering.

A scaffold, which can provide mechanical support for tissue regeneration and simultaneously release functionally active biomolecules are highly desirable for tissue engineering applications. Herein, we report the fabrication of a fibrous mesh of polycaprolactone (PCL) incorporating PCL-pluronic (F127) microvesicles through electrospinning, by exploiting the slow dissolution of PCL in glacial acetic acid (g-AA). Micro-vesicles 1-10 μm in diameter were fabricated through a non-solubility driven spontaneous self-assembly and stabilization of F127 with low molecular weight PCL in tetrahydrofuran-water mixture.