Publications by authors named "Dhammika Gunasekera"
Background: There is a shortage of rapid, accurate, and low-cost assays for diagnosing enteric fever. The dual-path platform for typhoid (DPPT) assay had high accuracy in retrospective studies with banked plasma samples. We aimed to evaluate the diagnostic accuracy of the DPPT assay in a prospective study using fingerstick capillary blood.
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- There is a significant need for a reliable point-of-care diagnostic test for typhoid, and recent research focused on two specific antigens (LPS and HlyE) that help differentiate typhoid from other infections.
- A study evaluated the Dual Path Platform (DPP) Typhoid assay's effectiveness using archived serum samples, showing a sensitivity of 97.8% and specificity of 65.3% at manufacturer's thresholds, with improved overall accuracy at optimal thresholds.
- The DPP Typhoid assay outperformed other rapid diagnostic tests in the same study cohort, highlighting its potential for high diagnostic accuracy, although further adjustments to the testing thresholds are suggested to enhance its performance.
Background: Multicenter studies were conducted to evaluate the DPP(®) HIV 1/2 Assay using oral fluid (OF) and fingerstick (FS) specimens in two different countries at the point of care (POC).
Objective: To evaluate the DPP(®) HIV 1/2 Assay using OF and FS specimens when compared to various worldwide algorithms for the detection of HIV.
Methods: At each testing center, each participant was tested using the DPP HIV 1/2 Assay using OF and FS specimens.
Mammalian phosphofructokinase (PFK) has evolved by a process of tandem gene duplication and fusion to yield a protein that is more than double the size of prokaryotic PFKs. On the basis of complete conservation of active site residues in the N-terminal half of the eukaryotic enzyme with those of the bacterial PFKs, one assumes that the active site of the eukaryotic PFK is located in the N-terminal half. Again using sequence comparisons, the four allosteric ligand sites of mammalian PFK have been thought to arise from the duplicated catalytic and regulatory sites of the ancestral PFK.
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