Copper-Cobalt Nanozyme Mimicking Laccase for Sensitive Colorimetric Determination of Cannabidiol in Food and Cosmetics.

In colorimetric analysis, nanozymes are invaluable tools due to their simple production, long-lasting stability, and adaptable enzymatic activity, which enable them to induce changes in substrate color. In this study, a simple nanozyme-based colorimetric sensor was developed to detect cannabidiol (CBD) by using the laccase activity of the self-made MOF with copper and cobalt loading (Cu/Co@MOF) nanozyme, which was synthesized using a one-pot microwave method. The Cu/Co@MOF has the ability to catalyze the coupling reaction between 4-AP and various phenolic substrates, thereby converting colorless phenolic substrates into red substances.

Transcriptomic analysis reveals pathways underlying the multi-antibiotic resistance of Klebsiella pneumoniae.

Klebsiella pneumoniae, an opportunistic pathogen, is pervasively distributed across the world. Its escalating antibiotic resistance poses a serious threat to global public health. The mechanisms behind this resistance remain largely elusive.

Bioinformatics analysis of the antigenic epitopes of L7/L12 protein in the B- and T-cells active against Brucella melitensis.

The objective is to analyse the physicochemical properties, spatial structure and protein-protein interactions (PPIs) of L7/L12 protein using bioinformatics methods and predict their B- and T-cell epitopes to lay a theoretical foundation for developing a novel multiepitope vaccine (MEV). The National Center for Biotechnology Information (NCBI) database was searched for the amino acid sequences of L7/L12 from . In addition, the online softwares, ProtParam and ProtScale, were used to predict the physicochemical properties: NetPhos3.

Dual-mode colorimetric-fluorescence biosensor for endotoxin detection based on CS@Fe,Cu/CDs-MnO nanomaterials.

Endotoxins are found in the outer membrane of all gram-negative bacteria and are a primary cause of endotoxemia, organ failure, and significant harm to human health. Limulus reagent as traditional detection method has certain limitations for the rapid and accurate detection of endotoxin due to the high costs associated with the horseshoe crab used in the sensing process. Herein, a fluorometric and colorimetric dual-mode biosensor was prepared for the rapid and sensitive detection of endotoxin.

Late-Stage Rapid [F]Trifluoromethyl Radiolabeling of Terminal Alkenes at Room Temperature.

We report an efficient, metal-free method for late-stage rapid [F]trifluoromethyl radiolabeling of terminal alkenes at room temperature. Utilizing 3,3-difluoroallyl sulfonium salts as precursors, the process achieves high radiochemical yields (up to 94 ± 2%) in just 30 s, with excellent functional group tolerance. This method offers a simplified and efficient pathway to produce [F]trifluoromethylated terminal alkene compounds, enabling their application in PET imaging and expanding the chemical space for drug discovery.

Unraveling the Role of Contaminants Reshaping the Microflora in Zea mays Seeds from Heavy Metal-Contaminated and Pristine Environment.

Heavy metal (HM) contaminants are the emerging driving force for reshaping the microflora of plants by eradicating the non-tolerance and non-resistant microbes via their lethal effects. Seeds served as a prime source of ancestral microbial diversity hereditary transfer from generation to generation. However, the problem arises when they got exposed to metal contamination, does metal pollutant disrupt the delicate balance of microbial communities within seeds and lead to shifts in their microflora across generations.

Complete genome sequence of strain DY294, a strain of isolated from Arshan Resort in Hulunbuir.

The Arshan Resort is a unique mineral spring whose water is used in traditional Mongolian medicine to treat many kinds of diseases. Here, we report the genome sequence of Strain DY294, a strain isolated from the Arshan water collected from Hulunbuir (117°40' E, 48°54' N), China.

Multielectron Reduction of Nitrosoarene via Aluminylene-Silylene Cooperation.

The cooperative effects of main-group elements pave the way for novel chemical transformations. However, the potential of bimetallic complexes featuring the most abundant aluminum and silicon elements remains largely unexplored. In this study, we present the synthesis and characterization of bis(silylene)-stabilized aluminylene .

MOF-mediated cascade reaction system for ultrasensitive detection of alkaline phosphatase activity and organophosphorus pesticides.

A metal-organic-framework (MOF) fluorescent sensor is reported based on NH-MIL-101(Fe) propelled pesticide and alkaline phosphatase (ALP) catalytic reaction. Different from previous reports, a cascade reaction system combined with MOF structural changes to generate fluorescence was employed. The rationale is that ALP can hydrolyze L-ascorbic acid 2-phosphate (AAP) into L-ascorbic acid (AA), which can reduce Fe to decompose structurally NH-MIL-101(Fe), resulting in 2-aminoterephthalic acid (NH-BDC) with intense fluorescence.

Ions-induced Assembly of Perovskite Nanocomposites for Highly Efficient Light-Emitting Diodes with EQE Exceeding 30.

Metal halide perovskites, a cost-effective class of semiconductos, hold great promise for display technologies that demand high-efficiency, color-pure light-emitting diodes (LEDs). Early research on three-dimensional (3D) perovskites showed low radiative efficiencies due to modest exciton binding energies. To inprove luminescence, reducing dimensionality or grain size has been a common approach.

Host Engineering of Deep-Blue-Fluorescent Organic Light-Emitting Diodes with High Operational Stability and Narrowband Emission.

The realization of highly operationally stable blue organic light-emitting diodes (OLEDs) is a challenge in both academia and industry. This paper describes the development of anthracene-dibenzofuran host materials, 2-(10-(naphthalen-1-yl)anthracen-9-yl)naphtho[2,3-b]benzofuran (Host 1) and 2-(10-([1,1'-biphenyl]-2-yl)anthracen-9-yl)naphtho[2,3-b]benzofuran (Host 2), namely for use in the emissive layer of an OLED stack. A multiple-resonance thermally activated delayed serves as the blue fluorescence emitter and exhibits an initial luminance of 1000 cd m and long operational stability (i.

Direct C3-H Alkylation and Alkenylation of Quinolines with Enones.

Conversion of quinoline C-H bonds into C-C bonds is essential for obtaining the enormous array of derivatives required for pharmaceutical and agrochemical development. Despite over a century of synthetic efforts, direct alkylation and alkenylation at C3-H positions in a wide array of quinoline precursors remain predominantly challenging and elusive. This report outlines the first successful quinoline C3-H alkylation and alkenylation reactions, exhibiting exceptional regio- and stereoselectivity, all achieved under redox-neutral and transition-metal-free conditions.

Carbon dots based AuAg@AuNPs with oxidase-like activity for SERS dual-readouts detection of D-penicillamine.

An oxidase (OXD) -like AuAg@AuNPs nanozyme was prepared by Au seeds growth using dopamine carbon dots as reducing and capping agents. The AuAg@AuNPs show excellent OXD-like and surface-enhanced Raman spectroscopy (SERS) activities and can oxidize the non-Raman-active leucomalachite green (LMG) into the Raman-active malachite green (MG). The research displays that D-penicillamine (D-PA) can effectively inhibit the OXD-like activity of Au@AgNPs and enhance the SERS signals as substrate.

Fluorescent/colorimetric probe for the detection of Cr(Ⅵ) based on MIL-101(Fe)-NH with peroxidase-like activity.

In the present research, Fe-based metal-organic frameworks (MIL-101(Fe)-NH) nanoparticles were synthesized by simple solvothermal methods and used to assay Cr(Ⅵ). The MIL-101(Fe)-NH performs dual functions: the 2-aminoterephthalic acid (NH-BDC) ligand endows a strong fluorescence emission, and the Fe metal nodes are able to facilitate the oxidation of 3,3',5,5'- tetramethylbenzidine (TMB) directly, resulting in the generation of oxidized-TMB (ox-TMB). Our research results showed that reducing agents such as ascorbic acid (AA) can collapse the structures of MIL-101(Fe)-NH because of the reduction of Fe by AA, resulting in release of NH-BDC.

Asymmetric Structural Engineering of Hot-Exciton Emitters Achieving a Breakthrough in Non-Doped BT.2020 Blue OLEDs with a Record 9.5% External Quantum Efficiency.

High-efficiency non-doped deep-blue organic light-emitting diodes (OLEDs) meeting the standard of BT.2020 color gamut is desired but rarely reported. Herein, an asymmetric structural engineering based on crossed long-short axis (CLSA) strategy is developed to obtain three new deep-blue emitters (BICZ, PHDPYCZ, and PHPYCZ) with a hot-exciton characteristic.

A colorimetric platform for sensitive sensing of Hg and S based on Se-AuNPs with Hg-activated peroxidase-like activity.

Herein, the selenium (Se) modified gold nanoparticles (Se-AuNPs) was synthesized using cerium doped carbon dots (Ce-CDs) as a reducing agent and template. As desired, Se-AuNPs displays enhanced peroxidase (POD)-like activity in the presence of Hg. The mechanism for the enhanced activity was attributed to the increased affinity between Se-AuNPs-Hg and the substrate, in which Se and Au elements have a strong binding capacity to Hg, forming Hg-Se bonds and Au-Hg amalgam to generate more ·OH.

Fluorescence detection of valence speciation of Cr(III) based on the catechol oxidase mimic enzyme activity of CuSeNP nanozymes.

Copper selenide nanoparticles (CuSeNP) were synthesized using histidine, ethylenediamine, and sodium selenate as precursors by one-step microwave digestion methods. The as-prepared CuSeNPs exhibit excellent catechol oxidase mimic enzyme and catalase (CAT)-like activities. Dopamine (DA) can be oxidized to aminochrome with HO by CuSeNPs, and the intermediate product aminochrome can further react with α-naphthol to yield a highly fluorescent derivative.

Integration of Gut Microbiota, Serum Metabolomic, and Network Pharmacology to Reveal the Anti Insomnia Mechanism of Mongolian Medicine Sugemule-4 Decoction on Insomnia Model Rats.

Objective: To explored the potential molecular mechanism of Sugemule-4 decoction (MMS-4D) in treating insomnia.

Methods: -4-chlorophenylalanine (PCPA) + chronic unpredictable mild stress stimulation (CUMS) was used to induce an insomnia model in rats. After the model was successfully established, MMS-4D was intervened at low, medium, and high doses for 7 days.

Cu,Zn,I-Doped Carbon Dots with Boosted Triple Antioxidant Nanozyme Activity for Treatment of DSS-Induced Colitis.

Nanozyme-mediated antioxidative therapy is a promising star for treating a myriad of important diseases through eliminating excessive reactive oxygen species (ROS) such as O and HO, a critical mechanism for inflammatory bowel disease (IBD). This work provides a high biocompatibility iodine-copper-zinc covalent doped carbon dots (Cu,Zn,I-CDs) with the catalase (CAT)-, superoxide dismutase (SOD)- and glutathione peroxidase (GPx)-like catalytic activities for treating ulcerative colitis (UC) by scavenging overproduced ROS. We found that I dopant aids in counteracting the positive charge at Cu,Zn dopants brought on by low pH, enabling Cu,Zn,I-CDs to process strong triple antioxidant nanozyme activities rather than Cu,Zn-CDs.

Colorimetric-SERS dual-mode aptasensor for Staphylococcus aureus based on MnO@AuNPs oxidase-like activity.

The nanozyme-linked aptamer-sorbent assay (NLASA) is a rapid way to screen and characterize aptamer binding to targets. In this paper, a MnO@AuNPs@aptamer (Apt) based NLASA coupled with colorimetric-SERS dual-mode for Staphylococcus aureus (S. aureus) detection is presented.

Aptamer-functionalized FeO/MWCNTs@Mo-CDs nanozyme for rapid colorimetric detection toward Escherichia coli.

The pathogenic bacteria induced foodborne disease has been detrimental to public health worldwide. Herein, the peroxidase (POD)-like FeO/MWCNTs@Mo-CDs (FMMC) nanozyme was applied for the detection of Escherichia coli (E. coli).

New Cocrystals of Ligustrazine: Enhancing Hygroscopicity and Stability.

Ligustrazine (TMP) is the main active ingredient extracted from , which is used in the treatment of cardiovascular and cerebrovascular diseases, with the drawback of being unstable and readily sublimated. Cocrystal technology is an effective method to improve the stability of TMP. Three benzoic acid compounds including P-aminobenzoic acid (PABA), 3-Aminobenzoic acid (MABA), and 3,5-Dinitrobenzoic acid (DNBA) were chosen for co-crystallization with TMP.

A Red-Emission Fluorescent Probe with Large Stokes Shift for Detection of Viscosity in Living Cells and Tumor-Bearing Mice.

Abnormal viscosity is closely related to the occurrence of many diseases, such as cancer. Therefore, real-time detection of changes in viscosity in living cells is of great importance. Fluorescent molecular rotors play a critical role in detecting changes in cellular viscosity.

Purely Organic Room-Temperature Phosphorescence Molecule for High-Performance Non-Doped Organic Light-Emitting Diodes.

Purely organic molecules with room-temperature phosphorescence (RTP) are potential luminescent materials with high exciton utilization for organic light-emitting diodes (OLEDs), but those exhibiting superb electroluminescence (EL) performances are rarely explored, mainly due to their long phosphorescence lifetimes. Herein, a robust purely organic RTP molecule, 3,6-bis(5-phenylindolo[3,2-a]carbazol-12(5H)-yl)-xanthen-9-one (3,2-PIC-XT), is developed. The neat film of 3,2-PIC-XT shows strong green RTP with a very short lifetime (2.