A Klebsiella-phage cocktail to broaden the host range and delay bacteriophage resistance both in vitro and in vivo.

Bacteriophages (phages), viruses capable of infecting and lysing bacteria, are a promising alternative for treating infections from hypervirulent, antibiotic-resistant pathogens like Klebsiella pneumoniae, though narrow host range and phage resistance remain challenges. In this study, the hypervirulent K. pneumoniae NTUH-K2044 was used to purify phage ΦK2044, while two ΦK2044-resistant strains were used to purify two further phages: ΦKR1, and ΦKR8 from hospital sewage.

Synergy of bacteriophage depolymerase with host immunity rescues sepsis mice infected with hypervirulent of capsule type K2.

The hypervirulent (hvKp) with K1 and K2 capsular types causes liver abscess, pneumonia, sepsis, and invasive infections with high lethality. The presence of capsular polysaccharide (CPS) resists phagocytic engulfment and contributes to excessive inflammatory responses. Bacteriophage depolymerases can specifically target bacterial CPS, neutralizing its defense.

Coexistence of plasmid-mediated , , and in .

is a potential pathogen that has not been studied comprehensively. The emergence of multidrug-resistant (MDR) , specifically strains resistant to tigecycline and carbapenem, presents a significant challenge to clinical treatment. This investigation aimed to characterize MDR strain FK8966, co-carrying , , and by plasmids.

Plumbagin enhances antimicrobial and anti-biofilm capacities of chlorhexidine against clinical while reducing resistance mutations.

With the widespread misuse of disinfectants, the clinical susceptibility of () to chlorhexidine (CHX) has gradually diminished, posing significant challenges to clinical disinfection and infection control. employs overexpression of efflux pumps and the formation of thick biofilms to evade the lethal effects of CHX. Plumbagin (PLU) is a natural plant extract that enhances membrane permeability and reduces proton motive force.

Recent Progress of Multifunctional Molecular Probes for Triple-Negative Breast Cancer Theranostics.

Breast cancer (BC) poses a significant threat to women's health, with triple-negative breast cancer (TNBC) representing one of the most challenging and aggressive subtypes due to the lack of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) expression. Traditional TNBC treatments often encounter issues such as low drug efficiency, limited tumor enrichment, and substantial side effects. Therefore, it is crucial to explore novel diagnostic and treatment systems for TNBC.

Host immunity involvement in the outcome of phage therapy against hypervirulent infections.

Highly encapsulated hypervirulent (hvKp) causes severe infections. Bacteriophage therapy, an antibiotic alternative, effectively treats bacterial infections. Phage φFK1979 encoding polysaccharide depolymerases can target and disarm the capsule of hvKp FK1979, showing promise against FK1979 infection.

Impact of LuxS on virulence and pathogenicity in exhibiting varied mucoid phenotypes.

How the LuxS/AI-2 quorum sensing (QS) system influences the pathogenicity of is complicated by the heterogeneity of the bacterial mucoid phenotypes. This study aims to explore the LuxS-mediated regulation of the pathogenicity of with diverse mucoid phenotypes, including hypermucoid, regular-mucoid, and nonmucoid. The wild-type, knockout, and complemented strains of three clinical isolates with distinct mucoid phenotypes were constructed.

Azomycin Orchestrate Colistin-Resistant Complex's Colistin Resistance Reversal In Vitro and In Vivo.

The complex (ECC) is a group of nosocomial pathogens that pose a challenge in clinical treatment due to its intrinsic resistance and the ability to rapidly acquire resistance. Colistin was reconsidered as a last-resort antibiotic for combating multidrug-resistant ECC. However, the persistent emergence of colistin-resistant (COL-R) pathogens impedes its clinical efficacy, and novel treatment options are urgently needed.

The Properties of Linezolid, Rifampicin, and Vancomycin, as Well as the Mechanism of Action of Pentamidine, Determine Their Synergy against Gram-Negative Bacteria.

Combining pentamidine with Gram-positive-targeting antibiotics has been proven to be a promising strategy for treating infections from Gram-negative bacteria (GNB). However, which antibiotics pentamidine can and cannot synergize with and the reasons for the differences are unclear. This study aimed to identify the possible mechanisms for the differences in the synergy of pentamidine with rifampicin, linezolid, tetracycline, erythromycin, and vancomycin against GNB.

Clinical impact of the type VI secretion system on clinical characteristics, virulence and prognosis of Acinetobacter baumannii during bloodstream infection.

The type VI secretion system (T6SS) has been regarded as a late-model virulence factor widely distributed in Acinetobacter baumannii (A. baumannii). This study aimed to elucidate the clinical manifestations, the genetic background and microbiological characteristics of A.

A novel fast method for identifying the origin of Maojian using NIR spectroscopy with deep learning algorithms.

Maojian is one of China's traditional famous teas. There are many Maojian-producing areas in China. Because of different producing areas and production processes, different Maojian have different market prices.

Raman spectroscopy combined with machine learning algorithms to detect adulterated Suichang native honey.

Zhejiang Suichang native honey, which is included in the list of China's National Geographical Indication Agricultural Products Protection Project, is very popular. This study proposes a method of Raman spectroscopy combined with machine learning algorithms to accurately detect low-concentration adulterated Suichang native honey. In this study, the native honey collected by local beekeepers in Suichang was selected for adulteration detection.

Recognition of chronic renal failure based on Raman spectroscopy and convolutional neural network.

Purpose: Chronic renal failure (CRF) is a disease with a high morbidity rate that can develop into uraemia, resulting in a series of complications, such as dyspnoea, mental disorders, hypertension, and heart failure. CRF may be controlled clinically by drug intervention. Therefore, early diagnosis and control of the disease are of great significance for the treatment and prevention of chronic renal failure.