Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings.

Background: The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. In planta analyses of this dynamic process have typically been limited to only two organelles or proteins at a time in only a few cell types.

Results: We generated a transgenic Arabidopsis plant that contains four spectrally different fluorescent proteins.