Treatment of human sperm with GYY4137 increases sperm motility and resistance to oxidative stress.

Hydrogen sulfide (HS) has been shown to play a significant role in oxidative stress across various tissues and cells; however, its role in sperm function remains poorly understood. This study aimed to investigate the protective effect of GYY4137, a slow-releasing HS compound, on sperm damage induced by HO. We assessed the effects of GYY4137 on motility, viability, lipid peroxidation and caspase-3 activity in human spermatozoa in vitro following oxidative damage mediated by HO.

Effect of repeated vitrification of human embryos on pregnancy and neonatal outcomes.

Background: Repeated cryopreservation of embryos should occasionally be considered when embryos were not suitable for transfer. The effect of re-cryopreservation on embryos remains contentious.

Methods: This retrospective cohort study aimed to evaluate the pregnancy and neonatal outcomes of twice vitrificated blastocyst derived from once vitrified embryos.

Oxidative stress and inflammatory markers in ovarian follicular fluid of women with diminished ovarian reserve during in vitro fertilization.

Background: Follicular microenvironment has been proposed as an important factor for oocyte grown and maturation. We sought to evaluate the oxidative stress and inflammatory levels in follicular fluid (FF) and association with embryo quality in patients with diminished ovarian reserve (DOR).

Methods: The current research included 46 DOR cases and 56 normal ovarian reserve (NOR) cases.

Developing Ganoderma lucidum as a next-generation cell factory for food and nutraceuticals.

Ganoderma lucidum holds a colossal reservoir of hydrolytic enzymes and therapeutic compounds and can be a sustainable source of proteins and bioactive compounds. Its metabolic versatility, propelled by its rich genome content, provides excellent biosynthetic machinery for innovation-driven pathway engineering. However, robust regulatory networks and low frequency of homologous recombination are critical bottlenecks that limit the development of molecular tools and precise genetic markers for biomanufacturing innovations in this organism.

Advanced genome-editing technologies enable rapid and large-scale generation of genetic variants for strain engineering and synthetic biology.

Targeted genome editing not only improves our understanding of fundamental rules in life sciences but also affords us versatile toolkits to improve industrially relevant phenotypes in various host cells. In this review, we summarize the recent endeavor to develop efficient genome-editing tools, and emphasize the utility of these tools to generate massive scale of genetic variants. We categorize these tools into traditional recombination-based tools, and more advanced CRISPR as well as RNA-based genome-editing tools.

Yiqixue buganshen recipe regulates the expression of integrin ανβ3 in the endometrium of controlled ovarian hyperstimulation mice.

Objective: To observe the effect of Yiqixue Buganshen recipe(, YBR) on the expression of integrin ανβ3 in the endometrium of controlled ovarian hyperstimulation mice.

Methods: A total of 180 mice were divided into three groups: model group, treatment group and control group. The treatment and model groups were intraperitoneally injected with gonadotropin-releasing hormone analogue for 7 days; pregnant mare serum gonadotropin was also injected on the 7th day.

[Fertilization method for primary infertility patients without definite cause undergoing in vitro fertilization and embryo transfer].

Objective: To determine the optimal fertilization method for primary infertility patients without definite causes undergoing in vitro fertilization and embryos transfer (IVF-ET).

Methods: A total of 321 IVF-ET cycles for primary infertility without definite causes were divided into two groups, namely group A with infertility period ≥ 5 years (165 cycles) and group B with infertility period <5 years (156 cycles). Each group was further divided into IVF, ICSI, and partial ICSI subgroups.

[Fertilization rates following 109 half intracytoplasmic sperm injection cycles for in vitro fertilization-embryo transfer].

Objective: To evaluate the clinical outcomes of half intracytoplasmic sperm injection (partial ICSI) treatment in infertile patients with potential fertilization failure.

Methods: A total of 109 partial ICSI cycles of in vitro fertilization-embryo transfer (IVF-ET) were classified into 5 groups, namely group A (infertile patients for unidentified causes, 17 cycles), group B (oligo-asthenozoospermia patients, 28 cycles), group C (teratozoospermia patients, 8 cycles), group D (primary infertile patients without definite causes, 31 cycles), and group E (secondary infertile patients without definite causes, 25 cycles). The fertilization rate and normal fertilization rate after IVF and ICSI were compared between the groups.