Urban ecology, stakeholders and the future of ecology.

The world human population is more and more urban and cities have a strong impact on the biosphere. This explains the development of urban ecology. In this context, the goal of our work is fourfold: to describe the diversity of scientific questions in urban ecology, show how these questions are organized, to assess how these questions can be built in close interactions with stakeholders, to better understand the role urban ecology can play within ecological sciences.

Differences in collembola species assemblages (Arthropoda) between spoil tips and surrounding environments are dependent on vegetation development.

Spoil tip production is one of the most extreme means of soil destruction, replacing the native soil with a coarse substrate. In this paper, we aim to determine the colonization of soil biota in new substrates, using collembola assemblages as an indicator. In Northern France, we sampled collembola communities in 11 coal mine spoil tips and their surroundings divided in four stages of vegetation development: bare soil, meadow, shrub and tree covers.

Regulatory mechanisms of group distributions in a gregarious arthropod.

In a patchy environment, how social animals manage conspecific and environmental cues in their choice of habitat is a leading issue for understanding their spatial distribution and their exploitation of resources. Here, we experimentally tested the effects of environmental heterogeneities (artificial shelters) and some of their characteristics (size and fragmentation) on the aggregation process of a common species of terrestrial isopod (Crustacea). One hundred individuals were introduced into three different heterogeneous set-ups and in a homogeneous set-up.

Evidence of self-organization in a gregarious land-dwelling crustacean (Isopoda: Oniscidea).

How individuals modulate their behavior according to social context is a major issue in the understanding of group initiation, group stability and the distribution of individuals. Herein, we investigated the mechanisms of aggregation behavior in Porcellio scaber, a terrestrial isopod member of the Oniscidea, a unique and common group of terrestrial crustaceans. We performed binary choice tests using shelters with a wide range of population densities (from 10 to 150 individuals).

Body shape in terrestrial isopods: A morphological mechanism to resist desiccation?

Woodlice are fully terrestrial crustaceans and are known to be sensitive to water loss. Their half-ellipsoidal shapes represent simple models in which to investigate theoretical assumptions about organism morphology and rates of exchange with the environment. We examine the influence of surface area and mass on the desiccation rates in three eco-morphologically different species of woodlice: Oniscus asellus, Porcellio scaber, and Armadillidium vulgare.

Experimental study of Lucilia sericata (Diptera Calliphoridae) larval development on rat cadavers: Effects of climate and chemical contamination.

Household products such as bleach, gasoline or hydrochloric acid have been used to mask the presence of a cadaver or to prevent the colonization of insects. These types of chemicals affect insect development and alter the forensic entomology analysis. This study was designed to test the effects of six household products (bleach, mosquito repellent, perfume, caustic soda, insecticide and unleaded gasoline) on blowfly (Lucilia sericata, Diptera: Calliphoridae) larval development.

Do necrophagous blowflies (Diptera: Calliphoridae) lay their eggs in wounds?: Experimental data and implications for forensic entomology.

This study was designed to examine the common belief that necrophagous blowflies lay their eggs in wounds. The egg-laying behaviour of Lucilia sericata was observed under controlled conditions on wet, artificially wounded or short-haired areas of rat cadavers. Flies laid significantly more eggs on the wet area and the area with short hair than on the dry area or area with long hair.

In vitro effects of household products on Calliphoridae larvae development: implication for forensic entomology.

Several parameters can delay the first arrival of flies on a corpse and the subsequent development of the larvae. This study focuses on the development of Lucilia sericata (Diptera: Calliphoridae) (Meigen, 1826) on household chemical-contaminated substrates. bleach, perfume, hydrochloric acid, caustic soda, insecticide, mosquito repellent, and gasoline in quantities consistent with an amount that could possibly be spilled on a corpse were mixed with beef liver to simulate contaminated fleshes.

The differential response of workers and queens of the ant Lasius niger to an environment marked by workers: ants dislike the unknown.

It is well known that ants can use cuticular hydrocarbons (CHCs) as a specific recognition cue. Most previous studies addressed the perception of CHCs occurring on the cuticle. However, the presence of CHCs in the environment (e.

Aggregation in woodlice: social interaction and density effects.

Terrestrial isopods are known to be sensitive to humidity, brightness or temperature. Until now, aggregation was assumed to depend on these sensitivities as a result of individual preferences. In this paper, we show that the social component is also important in the isopod aggregation phenomenon.

Individual preferences and social interactions determine the aggregation of woodlice.

Background: The aggregation of woodlice in dark and moist places is considered an adaptation to land life and most studies are focused on its functionality or on the behavioural mechanisms related to the individual's response to abiotic factors. Until now, no clear experimental demonstration was available about aggregation resulting from inter-attraction between conspecifics.

Methodology/main Findings: We present the dynamics of aggregation, not previously described in detail in literature, as being independent of the experimental conditions: homogeneous and heterogeneous environments with identical or different shelters.

Fos protein expression induced by intracerebroventricular injection of vasopressin in unconditioned and conditioned mice.

Arginine8-vasopressin (AVP) has been shown to improve memory consolidation in various mnemonic tasks. Our previous studies have pointed out the involvement of the hippocampus in memory consolidation and retrieval processes during discriminative learning by mice. The present study attempts to determine what other brain areas besides the hippocampus might be involved in the enhancing effect of intracerebroventricularly (i.

The behavioral effect of vasopressin in the ventral hippocampus is antagonized by an oxytocin receptor antagonist.

[Arg8]vasopressin improved long-term retrieval processes and relearning in a go-no go visual discrimination task when bilaterally microinjected at a dose of 25 pg/animal into the ventral hippocampus of mice, 10 min prior to the retention session. We had shown that this enhancing effect is antagonized by pretreatment with equal or lower doses (25 pg or 1 ng) of the vasopressin V1 receptor antagonist, (d(CH2)5Tyr(Me)-vasopressin). The present study was an attempt to determine whether the vasopressin V2 receptor antagonist or oxytocin receptor antagonist is as effective as the vasopressin V1 receptor antagonist to block the behavioral effect of vasopressin in the ventral hippocampus.

Effects of arginine8-vasopressin administered at different times in the learning of an appetitive visual discriminative task in mice.

A visual discrimination task was used to investigate the effect of the intra-hippocampal injection of arginine8-vasopressin (AVP) in male Balb/c mice at different stages of the learning processes. The peptide was bilaterally microinjected at a dose of 25 pg per animal, i.e.

Neonatal gamma-ray irradiation impairs learning and memory of an olfactory associative task in adult rats.

Adult neonatally gamma-irradiated rats were compared with control animals in a non-spatial olfactory associative task using two different procedures. Irradiation induced a clear reduction in the total mean area of the olfactory bulbs and hippocampus but not of the orbital prefrontal cortex, diagonal band and cell layers of the entorhinal and piriform cortex. The gamma-irradiation affected the granule cells of the olfactory bulbs and differentially altered the cell layers of the subfields of the ammonic fields and the dorsal and ventral blades of the dentate gyrus.

Distribution of cholecystokinin immunoreactivity in the BALB/c mouse forebrain: an immunocytochemical study.

The present study describes cholecystokinin (CCK) immunoreactivity (CCK-IR) distribution in the brains of control and colchicine-treated mice. In the brains of control mice, the CCK-IR strongly revealed numerous axons and terminals. Perikarya exhibiting a faint to moderate immunoreactivity were also observed in areas such as cortices, hippocampus, amygdala, septum, and thalamus.

Inhibition of the vasopressin-enhancing effect on memory retrieval and relearning by a vasopressin V1 receptor antagonist in mice.

We have previously shown that [Arg8]vasopressin bilaterally administered into the ventral hippocampus of mice at a dose of 0.025 ng/animal 10 min prior to the retention session, improved long-term retrieval processes and relearning of a Go-No-Go visual discrimination task. The purpose of the present study was to determine whether the vasopressin V1 receptor antagonist, -beta-mercapto-beta,beta-cyclopentamethylenepropionyl1, O-Me-Tyr2,Arg8]vasopressin, d(CH2)5Tyr(Me)vasopressin), is able to block the behavioral effect of arginine-vasopressin in the ventral hippocampus.

Effect of changes in the intrahippocampal vasopressin on memory retrieval and relearning.

Previous results have indicated the involvement of the hippocampus in the behavioral effect of vasopressin, with a better effect when the peptide was injected in the ventral part rather than in the dorsal part of this structure. The purpose of the present study was to determine, in mice, whether the injection of vasopressin or vasopressin antisera into the ventral hippocampus has an effect on retrieval and relearning of a Go-No Go visual discrimination task and, if so, to what extent this involvement of the vasopressin system depends on the integrity of the medial amygdaloid nucleus, the main source of vasopressin innervation in the ventral hippocampus in rats. In the first experiment, we showed that pretest microinjection of Arg8-vasopressin (25 pg per animal) in the ventral hippocampus alleviated forgetting observed after a prolonged interval of 24 days between the acquisition of information and its retrieval.

Effects of anti-CCK-8 antiserum on acquisition and retrieval by mice in an appetitive task.

Endogenous cholecystokinin (CCK) was blocked in the posterior cingulate cortex of BALB/c mice using a local injection of anti-CCK-8 antiserum, and memory effects were tested using Go-No Go visual discrimination conditioning. Injection of 0.4 microliter of anti-CCK-8 antiserum diluted to 1:10, 10-15 min before each session, produced substantial learning impairment on the discrimination task.

Effects of bilateral lesions of the cerebellar interpositus nucleus on the conditioned forelimb flexion reflex in mice.

Three groups of mice, unoperated controls, sham and lesioned, were submitted to an associative conditioning of forelimb flexion reflex (FFR). Light and tone constituted the conditioned stimulus (CS) paired with a forelimb electric shock, the unconditioned stimulus (UCS). The first two groups were able to acquire an appropriate conditioned response.

Microinjection of anti-vasopressin serum into hippocampus in mice: effects on appetitively reinforced task after intraventricular administration of Arg-vasopressin.

Antiserum to [Arg8]vasopressin (anti-AVP) was bilaterally administered into dorsal hippocampus at 1:50 or 1:10 dilution 20 min before the 24-day retention session of a visual discrimination task. This treatment by itself did not affect the retention performance by comparison with the respective control group, whatever the dilution of anti-AVP, suggesting that hippocampal endogenous AVP is not involved in our behavioral paradigm. On the other hand, intracerebroventricular (i.

Hippocampal lesions block behavioral effects of central but not of peripheral pre-test injection of arginine vasopressin in an appetitive learning task.

The effects of peripheral and central administration of arginine-vasopressin (AVP) were examined in intact or hippocampal-lesioned mice, when administered just before the retention session of an appetitive visual discrimination task. All the subjects underwent a partial learning of this task and were tested 24 days later when a partial forgetting occurred in controls. In Expt.

Age-related changes in the C57BL/6J mouse cochlea. II. Ultrastructural findings.

The C57BL/6J mouse organ of Corti was studied using transmission electron microscopy. The basal coil of the cochlea was examined in mice 1-50 days of age. At birth the cochlea was very immature but both types of hair cells were innervated.

Significance of presynaptic formations in early stages of cochlear synaptogenesis.

Very early stages of cochlear synaptogenesis are described in inner (IHCs) and outer hair cells (OHCs) of cat foetuses. Ten days before birth (DBB) well-formed synaptic contacts were found between afferent dendrites and both IHCs and OHCs. At the IHC level a preliminary stage before functioning has been proposed.