Profiling the membrane proteome of Shewanella oneidensis MR-1 with new affinity labeling probes.

The membrane proteome plays a critical role in electron transport processes in Shewanella oneidensis MR-1, a bacterial organism that has great potential for bioremediation. Biotinylation of intact cells with subsequent affinity-enrichment has become a useful tool for characterization of the membrane proteome. As opposed to these commonly used, water-soluble commercial reagents, we here introduce a family of hydrophobic, cell-permeable affinity probes for extensive labeling and detection of membrane proteins.

Quantification and characterisation of cyclo-oxygenase and lipid peroxidation inhibitory anthocyanins in fruits of Amelanchier.

The levels of bioactive anthocyanins in the fruits of Amelanchier alnifolia, A. arborea and A. canadensis have been determined by HPLC.

Spectroscopic and Photophysical Characterization of Fluorescent Chemosensors for Monosaccharides Based on -Phenylboronic Acid Derivatives of 1,8-Naphthalimide.

Spectroscopic and photophysical properties of two fluorescent probes for monosaccharides are presented. Probes are based on the -phenyl-1,8-naphthalimide structure having the boronic acid group [R-B(OH)] in in one case, and in the other case, positions of the -phenyl group. Formation of the anionic form of the boronic acid group [ ] induced a substantial decrease of the steady-state fluorescence of both compounds.