Enhanced-latex-agglutination assay for C-reactive protein in serum, with use of a centrifugal analyzer.

This is an improved assay of C-reactive protein in serum, for use with the Baker "Encore" centrifugal analyzer. Features of this assay include: 250-specimen throughput per hour, within-batch CV 2.2%, between-batch CV 2.

Insulin dependence: problems with the classification of 100 consecutive patients.

We have evaluated the clinical and immunogenetic features of 100 consecutive patients presenting to an adult diabetic clinic who were judged clinically to need insulin therapy but were not sufficiently ill to be admitted to hospital. Over this same period 15 newly diagnosed patients (aged 13-70 years) were started on insulin as in-patients of whom ten were in ketoacidosis. The 100 out-patients, aged 11-75 years at the time of starting insulin, were followed for at least a year.

C reactive protein concentrations in neonates: determination by a latex enhanced immunoassay.

A latex enhanced immunoassay on a centrifugal fast analyser was used to compare serum C reactive protein concentrations in maternal and neonatal blood. In the neonate the C reactive protein concentration at birth was less than 1.0 mg/l; the concentration rose slightly during the first two weeks of life.

Kinetics of the antigen:antibody reaction. Light scattering and particle size measurement using photon correlation spectroscopy.

Many techniques for the estimation of specific proteins measure light scattered by, or the turbidity of, suspensions of antigen:antibody aggregates formed in fluid-phase reactions. Although the shapes of reaction curves obtained are well documented, interpretation of the data in relation to the laws of light scattering and the mechanism of the antigen:antibody reaction are poorly described in the literature. Using photon correlation spectroscopy it has been shown that antigen:antibody complexes continue to grow in size even when no further increases in scatter intensity or turbidity are apparent.

Monoclonal antibodies to human IgG: reaction characteristics in the centrifugal analyzer.

We used monoclonal antibodies to human IgG in a kinetic turbidimetric method with a centrifugal analyzer. Results for total IgG with monoclonal and polyclonal reagents correlated well (r = 0.979).

Light scattering and absorption--developments in immunology.

Fluid-phase immunoprecipitation analysis has undergone two distinct paths of development. One path utilizes existing absorptiometric equipment while the other relies on apparatus constructed specifically for the measurement of scattered light. The principles and instrumentation employed in light scattering and absorption techniques are reviewed with special reference to their applications in immunology.

Quantitation of human total IgG, kappa IgG and lambda IgG in serum using monoclonal antibodies.

Monoclonal antibodies to human IgG have been applied, in parallel with polyclonal antisera, to the routine quantitation of human IgG. Two monoclonal antibodies directed against spatially distinct epitopes have been used in combination to form insoluble complexes exhibiting turbidity. Quantitation was performed using a centrifugal fast analyser and a correlation coefficient of 0.

Screening methods for covert bacteriuria in schoolgirls.

Screening tests for bacteriuria based on two different principles were evaluated in1582 schoolgirls aged 5-11 years, and in 26 girls aged 3-16 years attending hospitalwith symptomatic urinary tract infection. Tests for hypoglucosuria, performed by a semi-automated fluorometric method and with Uriglox strips on early-morning urine samples voided after overnight fasting, gave unacceptably high false-negative rates (16.7% and 20.

Bisalbuminaemia Birmingham - a new variant in an Indian Family. Characterisation and comparative studies with albumin Kashmir.

Bisalbuminaemia has rarely been reported in races of the Indian subcontinet. The variant described in a native of the Punjab is shown to have a similar electrophoretic mobility to albumin Kashmir but different dye binding properties. The dye binding properties are also different from other albumin variants where such studies have been reported.