Publications by authors named "Devendra Alrani"
Article Synopsis
- Oral verrucous lesions (OVLs) are challenging to diagnose due to varying histopathological features, and this study investigates Mcm-2 and Ki-67 as markers to help differentiate these lesions from oral squamous cell carcinoma (OSCC).
- Using 90 paraffin-embedded tissue samples, the researchers assessed the expression levels of Mcm-2 and Ki-67, alongside patient survival data, employing statistical methods for analysis.
- Findings revealed that Mcm-2 is a more sensitive and prognostic indicator than Ki-67, with higher overexpression in verrucous lesions and improved survival rates in verrucous squamous cell carcinoma (VSCC) compared to conventional OSCC, suggesting its potential for better diagnosis
Aim: The knowledge of cellular proteins that involves cell cycle and its control system is essential for understanding tumor biology. Minichromosome maintenance protein (Mcm-2), a component of prereplicative complex, essential for initiating DNA replication, is deregulated in different malignant lesions, and is expressed throughout the whole cell cycle including the G0 and G1 phases. This characteristic cell cycle event is not found in other proliferative markers such as geminin, AgNOR, Ki-67, and proliferating cell nuclear antigen.
View Article and Find Full Text PDFBackground: Mcm-2 is a biomarker belonging to Mcm family of proteins which has rarely been used in oral potentially malignant and malignant lesions of the verrucous type. The objective of this study is to assess the expression of Mcm-2 in Normal Oral Mucosa (NM), Verrucous Hyperplasia (VH), Verrucous Carcinoma (VC) and Oral Squamous Cell Carcinoma (OSCC) and compare it with the clinicopathological characteristics.
Methodology: A total of 70 formalin fixed paraffin embedded tissue samples (10 cases of Normal Mucosa NM- Group A, 10 cases of Verrucous Hyperplasia- VH without Dysplasia- Group B, 10 cases of Verrucous Hyperplasia- VH with Dysplasia- Group C, 20 cases of Verrucous Carcinoma VC-Group D, 20 cases of Oral Squamous Cell Carcinoma OSCC- Group E) were subjected to immunohistochemistry with Mcm-2 antibody.
Aim: The aim of the present study was to: (a) analyze the nature of collagen with respect to cohesive and discohesive invasive tumor front (ITF) of oral squamous cell carcinoma (OSCC); (b) analyze the clinicopathological correlation with the nature of collagen at the ITF of OSCC; and (c) correlate the nature of collagen with Broder's and Bryne's histological grading system of OSCC.
Methods: Tissue sections of 29 OSCC with ITF were stained with hematoxylin-eosin and picrosirius red staining for evaluation under a polarized microscope.
Results: Tumors with a cohesive front had a thick collagen fiber, are predominantly organized, red-yellow in color, well packed, and show strong birefringence (P<.