Drought-induced modifications of photosynthetic electron transport in intact leaves: analysis and use of neural networks as a tool for a rapid non-invasive estimation.

Water deficit is one of the most important environmental factors limiting sustainable crop yields and it requires a reliable tool for fast and precise quantification. In this work we use simultaneously recorded signals of photoinduced prompt fluorescence (PF) and delayed fluorescence (DF) as well as modulated reflection (MR) of light at 820nm for analysis of the changes in the photosynthetic activity in detached bean leaves during drying. Depending on the severity of the water deficit we identify different changes in the primary photosynthetic processes.

Preservation of integrity and activity of Haberlea rhodopensis photosynthetic apparatus during prolonged light deprivation.

The effect of prolonged light deprivation on ultrastructure, pigment composition and functions of photosynthetic apparatus of the resurrection plant Haberlea rhodopensis Friv. (Gesneriaceae) was studied. For this purpose, intact plants were kept in darkness for up to 6 months.

Relationship between the degree of carotenoid depletion and function of the photosynthetic apparatus.

Fluridone, an inhibitor of the carotenoid biosynthesis, was used to study the relationship between the degree of carotenoid depletion and the function of the photosynthetic apparatus. The data reveal that, at a small reduction of the carotenoid content (25% decrease of the total carotenoids), the PSII and PSI (oxidation of P700 by far-red light) photochemistry is not influenced, while the oxygen evolution is strongly inhibited. Further reduction of the total carotenoid content (more than 40%) leads to decrease of the chlorophyll content and inhibition of the functions of both photosystems as the effect on the photosynthetic oxygen evolution and primary photochemistry is stronger than the effect on P700 oxidation.

Preservation of photosynthetic electron transport from senescence-induced inactivation in primary leaves after decapitation and defoliation of bean plants.

The comparative effects of decapitation and defoliation on the senescence-induced inactivation of photosynthetic activity in primary leaves of bean plants were investigated. Decapitation was performed during different phases of bean plant ontogenesis, immediately after the appearance of the 1st, 2nd, 3rd and 4th composite leaf. In addition, we examined a variant with primary leaves and stem with an apical bud, but without composite leaves, i.

Non-photochemical loss in PSII in high- and low-light-grown leaves of Vicia faba quantified by several fluorescence parameters including L(NP), F0/F'm, a novel parameter.

Using the expression of fluorescence originated from the PSII open reaction center in the light by Oxborough and Baker (1997), we obtained a formula that expresses relationships between the quantum efficiency of PSII photochemistry in the dark (Phi(m)= F(v)/F(m)) and in the light Phi'(m)=F'(v)/F'(m):Phi'(m)=Phi(m)+L(NP), where L(NP)(=F(0)/F'(m)) denotes the quantum yield of light induced non-photochemical losses (heat dissipation and fluorescence de-excitation) in PSII. Using L(NP) and other conventional fluorescence parameters, we conducted quenching analyses with leaves of broad bean plants (Vicia faba L.) grown at 700 (high light; HL) and 80 mumol photons m(-2) s(-1) (low light; LL).

The symptomless leaf infection with grapevine leafroll associated virus 3 in grown in vitro plants as a simple model system for investigation of viral effects on photosynthesis.

The photosynthetic changes evaluated by oxygen evolution, chlorophyll fluorescence, photoacoustics, and delayed fluorescence (DF) were studied in leaves of grown in vitro for 8 weeks grapevine plants (Vitis vinifera) infected by grapevine leafroll-associated virus 3 (GLRaV-3). The infected leaves were characterized during the viral infection without visible disease symptoms. The symptomless infection led to a decrease in plant biomass.

Comparative study on the changes in photosynthetic activity of the homoiochlorophyllous desiccation-tolerant Haberlea rhodopensis and desiccation-sensitive spinach leaves during desiccation and rehydration.

The functional peculiarities and responses of the photosynthetic system in the flowering homoiochlorophyllous desiccation-tolerant (HDT) Haberlea rhodopensis and the non-desiccation-tolerant spinach were compared during desiccation and rehydration. Increasing rate of water loss clearly modifies the kinetic parameters of fluorescence induction, thermoluminescence emission, far-red induced P700 oxidation and oxygen evolution in the leaves of both species. The values of these parameters returned nearly to the control level after 24 h rehydration only of the leaves of HDT plant.