Effect of the ecto-ATPase inhibitor, ARL 67156, on the bovine chromaffin cell response to ATP.

Bovine chromaffin cells contain an ecto-ATPase (K(m)=1.57 +/- 0.27 x 10(-4) M) which can hydrolyze ATP present in the culture media.

Transduction of bovine adrenal chromaffin cells using a recombinant adenovirus expressing GFP.

Insertion of genetic material into bovine chromaffin cells employing various techniques have produced low to moderate transduction rates. Recent technology using adenoviral gene transfer has become one of the most powerful methods for introducing genes into mammalian cells. We examined whether a recombinant adenovirus could provide a convenient vector to transfer genes of interest for mechanistic studies on chromaffin cells.